Department of Biomathematics, Institute of Physiology Academy of Sciences of the Czech Republic, Prague, Czech Republic.
Physiol Res. 2014;63(Suppl 1):S49-55. doi: 10.33549/physiolres.932679.
3D microscopy and image analysis provide reliable measurements of length, branching, density, tortuosity and orientation of tubular structures in biological samples. We present a survey of methods for analysis of large samples by measurement of local differences in geometrical characteristics. The methods are demonstrated on the structure of the capillary bed in a rat brain.
3D 显微镜和图像分析为生物样本中管状结构的长度、分支、密度、曲折度和方向提供了可靠的测量方法。我们展示了一种通过测量局部几何特征差异来分析大样本的方法综述。该方法在大鼠脑毛细血管床的结构上得到了验证。
