Yoda K, Yasuda H, Jiang X W, Okazaki T
Department of Molecular Biology, School of Science, Nagoya University, Japan.
Nucleic Acids Res. 1988 Jul 25;16(14A):6531-46. doi: 10.1093/nar/16.14.6531.
Using DNA molecules synthesized in the early stage of lambda phage infection, deoxynucleotides at the transition sites from primer RNA to DNA synthesis have been mapped in the 1.5 kbase area of the lambda phage genome containing the genetically defined replication origin (ori lambda). Sites in the 1-strand (the polarity of the 1-strand is 5' to 3' from the left to the right direction of the lambda phage genetic map) were distributed both inside and outside of the ori lambda, whereas the sites in the r-strand (the strand in the opposite polarity) were mainly distributed more than three hundred nucleotides apart from the ori lambda to the right. A CPuPu sequence was found at -12 to -10 region of transition sites of the r- and the 1-strands in the frequency of 80% and 70%, respectively, and over 60% of the CPuPu sequences were CAG. Properties of the transition sites are discussed in relation to the primer synthesis.
利用在λ噬菌体感染早期合成的DNA分子，已在λ噬菌体基因组中包含遗传定义的复制起点（ori λ）的1.5千碱基区域内，对从引物RNA到DNA合成的过渡位点处的脱氧核苷酸进行了定位。1链（从λ噬菌体遗传图谱的左到右方向，1链的极性为5'至3'）上的位点分布在ori λ的内部和外部，而r链（极性相反的链）上的位点主要分布在从ori λ向右超过三百个核苷酸的位置。在r链和1链的过渡位点的-12至-10区域分别发现了频率为80%和70%的CPuPu序列，并且超过60%的CPuPu序列为CAG。结合引物合成讨论了过渡位点的特性。
