猫过敏原 Fel d 1 和非 Fel d 1 的 IgE 和 IgG 结合模式及 T 细胞识别。

IgE and IgG binding patterns and T-cell recognition of Fel d 1 and non-Fel d 1 cat allergens.

机构信息

Telethon Institute for Child Health Research and Centre for Child Health Research, University of Western Australia, Subiaco Wa, Australia.

出版信息

J Allergy Clin Immunol Pract. 2013 Nov-Dec;1(6):656-65.e1-5. doi: 10.1016/j.jaip.2013.08.008. Epub 2013 Oct 31.

DOI:10.1016/j.jaip.2013.08.008

PMID:24565714

Abstract

BACKGROUND

Cat allergy affects approximately 15% of the population and is a major risk factor for asthma. The relative importance of cat allergens other than Fel d 1 is not known.

OBJECTIVE

To compare IgE and IgG antibody binding and T-cell recognition of the major cat allergen Fel d 1 with other cat proteins with known IgE binding properties.

METHODS

IgE, IgG1, and IgG4 antibody to Fel d 1, 2, 3, 4, 7, 8, and the undesignated IgE binding proteins haptoglobin and S100A12 were measured in the plasma of 96 individuals with cat allergy and 78 individuals without cat allergy. Cytokines were measured from T cells stimulated with the cat allergens.

RESULTS

An allergen other than Fel d 1 had the highest IgE binding specificity for 35% of individuals with cat allergy, and it bound more than 50% of their IgE antibody in 70% of these sera. Fel d 4, 7, and 8 were identified as the main contributors to the non-Fel d 1 IgE binding response and elicited inflammatory Th2 cytokines to a similar degree as Fel d 1. As expected, the average percentage of IgE binding to Fel d 1 for individuals was 55%. IgG4 binding to Fel d 1 was detected in both subjects with allergy (30%) and subjects without allergy (19%). IgG4 binding to the other allergens was less prevalent but was found for both groups. IgG1 antibody was not detected to any of the newly described cat proteins.

CONCLUSION

Fel d 4, 7, and 8 are allergens that should be included in the diagnosis and investigation of cat allergy.

摘要

背景

猫过敏影响约 15%的人口，是哮喘的主要危险因素。除了 Fel d 1 之外，其他猫过敏原的相对重要性尚不清楚。

目的

比较主要猫过敏原 Fel d 1 与其他具有已知 IgE 结合特性的猫蛋白的 IgE 和 IgG 抗体结合和 T 细胞识别。

方法

在 96 名猫过敏患者和 78 名无猫过敏患者的血浆中测量了对 Fel d 1、2、3、4、7、8 以及未指定的 IgE 结合蛋白触珠蛋白和 S100A12 的 IgE、IgG1 和 IgG4 抗体。用猫过敏原刺激 T 细胞后测量细胞因子。

结果

除 Fel d 1 以外的过敏原对 35%的猫过敏患者具有最高的 IgE 结合特异性，在这些血清中，有 70%的患者其 IgE 抗体的结合量超过 50%。Fel d 4、7 和 8 被确定为非 Fel d 1 IgE 结合反应的主要贡献者，并与 Fel d 1 相似程度地引发炎症性 Th2 细胞因子。如预期的那样，个体对 Fel d 1 的 IgE 结合平均百分比为 55%。过敏患者（30%）和无过敏患者（19%）中均检测到 Fel d 1 的 IgG4 结合。其他过敏原的 IgG4 结合虽不常见，但在两组中均有发现。未检测到针对新描述的任何猫蛋白的 IgG1 抗体。