Baj Z, Tchórzewski H, Majewska E
Department of Pathophysiology, Institute of Basic Medical Sciences WAM, Lódź.
Allerg Immunol (Leipz). 1988;34(1):10-6.
Adherent PMNLs secrete protein factor termed granulocyte factor (GF). The antigenic and some structural features of GF were examined in the present paper. Molecular weight of GF was established on Sephadex G-75 column and is amounted to 40 kD. GF exhibited two isoelectric points in pH ranged from 4.7 to 4.9 and from 5.7 to 6.4. SDS polyacrylamide gel electrophoresis has displayed two bands, 20 and 68 kD, respectively. Initial analysis of amino acid composition of purified GF has shown 15 amino acids, but without aromatic and thioamino acids. Antigen-dependent species specificity was confirmed using rabbit anti-human, -murine, -guinea pig or-rat - GF IgG applying conventional precipitation methods in agar gel no crossreaction was noted. Using precipitation analyses GF was localized in the specific granules of PMNLs.
黏附的多形核中性粒细胞（PMNLs）分泌一种称为粒细胞因子（GF）的蛋白质因子。本文研究了GF的抗原特性和一些结构特征。通过葡聚糖凝胶G-75柱测定了GF的分子量，其为40kD。GF在pH值4.7至4.9和5.7至6.4范围内表现出两个等电点。十二烷基硫酸钠聚丙烯酰胺凝胶电泳分别显示出两条带，分子量分别为20kD和68kD。对纯化的GF的氨基酸组成进行初步分析显示有15种氨基酸，但不含芳香族和含硫氨基酸。使用兔抗人、抗小鼠、抗豚鼠或抗大鼠的GF IgG，通过常规琼脂凝胶沉淀法证实了抗原依赖性物种特异性，未发现交叉反应。通过沉淀分析发现GF定位于PMNLs的特异性颗粒中。
