Baj Z, Zeman K, Nowak Z, Majewska E, Tchórzewski H
Department of Pathophysiology, Institute of Basic Medical Sciences WAM, Lódz.
Folia Biol (Praha). 1988;34(6):390-8.
Activated polymorphonuclear leucocytes (PMNL) secrete a specific factor possessing numerous immunoregulatory properties. The rabbit IgG antibodies against this human granulocyte factor GF (anti-GF-IgG) have significantly changed the regulatory features of GF and the same antibodies alone have also exerted immunoregulatory potency when tested in vitro. Inhibition of mixed lymphocyte reaction (MLR) proliferation after GF treatment was abolished in the presence of anti-GF-IgG. GF displays a potentiating effect on interleukin-1 (IL-1) production by human monocytes in vitro. Anti-GF-IgG did not change the potentiating capacity of GF and these specific antibodies alone also enhanced IL-1 production to the same extent as GF. In peripheral blood mononuclear cell (PBMC) cytotoxicity test, the cytotoxic activity of effector cells was markedly reduced in the presence of GF. Anti-GF-IgG partially abrogated the observed suppression, although their own features indicate a strong inhibitory effect on PBMC cytotoxicity. These contradictory results are difficult to explain but they may enable us to understand the pleiotropic immunoregulatory mechanism of both GF and anti-GF-IgG. The results of the present and recently published studies support the idea that both GF and anti-GF-IgG modify T lymphocyte function at the same receptor level.
活化的多形核白细胞（PMNL）分泌一种具有多种免疫调节特性的特定因子。针对这种人粒细胞因子GF的兔IgG抗体（抗GF-IgG）显著改变了GF的调节特性，并且单独的相同抗体在体外测试时也发挥了免疫调节作用。在抗GF-IgG存在的情况下，GF处理后对混合淋巴细胞反应（MLR）增殖的抑制作用被消除。GF在体外对人单核细胞产生白细胞介素-1（IL-1）具有增强作用。抗GF-IgG没有改变GF的增强能力，并且单独的这些特异性抗体也能将IL-1的产生增强到与GF相同的程度。在外周血单核细胞（PBMC）细胞毒性试验中，在GF存在的情况下，效应细胞的细胞毒性活性明显降低。抗GF-IgG部分消除了观察到的抑制作用，尽管它们自身的特性表明对PBMC细胞毒性有强烈的抑制作用。这些相互矛盾的结果难以解释，但它们可能使我们能够理解GF和抗GF-IgG的多效性免疫调节机制。目前和最近发表的研究结果支持这样一种观点，即GF和抗GF-IgG在相同的受体水平上调节T淋巴细胞功能。
