Preparation of Arabidopsis thaliana seedling proteomes for identifying metacaspase substrates by N-terminal COFRADIC.

作者信息

Tsiatsiani Liana, Stael Simon, Van Damme Petra, Van Breusegem Frank, Gevaert Kris

机构信息

Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands.

出版信息

Methods Mol Biol. 2014;1133:255-61. doi: 10.1007/978-1-4939-0357-3_16.

DOI:10.1007/978-1-4939-0357-3_16

PMID:24567107

Abstract

Proteome-wide discovery of in vivo metacaspase substrates can be obtained by positional proteomics approaches such as N-terminal COFRADIC, for example by comparing the N-terminal proteomes (or N-terminomes) of wild-type plants to transgenic plants not expressing a given metacaspase. In this chapter we describe a protocol for the preparation of plant tissue proteomes, including differential isotopic labelling allowing for a comparison of in vivo N-terminomes that serves as the starting point for N-terminal COFRADIC studies.

摘要