Central Institute for Engineering, Electronics and Analytics, ZEA-3, Forschungszentrum Jülich, Jülich, Germany.
Department of Biomedicine, Aarhus University, Aarhus, Denmark.
Methods Mol Biol. 2022;2447:271-283. doi: 10.1007/978-1-0716-2079-3_22.
A large variety of enrichment procedures for protein N-termini have been developed to trace protease activity and determine precise cleavage sites, as well as other N-terminal protein modifications. Typically, enriched N-terminal peptides are identified by tandem mass spectrometry using standard database search engines, in many cases the popular MaxQuant software package. MaxQuant Advanced N-termini Interpreter (MANTI) is a software package that helps to validate, annotate, and visualize peptide identifications in N-termini datasets in a rapid and straightforward manner. Usage of MANTI and especially its graphical interface Yoğurtlu MANTI in detail are described to enable users to take full advantage of the software package and the multitude of options it has to offer.
已经开发了多种蛋白质 N 末端的富集程序，以追踪蛋白酶活性和确定精确的切割位点，以及其他 N 末端蛋白质修饰。通常，富集的 N 末端肽通过串联质谱分析，使用标准数据库搜索引擎，在许多情况下是流行的 MaxQuant 软件包进行鉴定。MaxQuant 高级 N 末端解释器（MANTI）是一个软件包，可帮助快速、直接地验证、注释和可视化 N 末端数据集的肽鉴定。详细描述了 MANTI 的使用方法，特别是其图形界面 Yoğurtlu MANTI，以使用户能够充分利用该软件包及其提供的多种选项。
