Wu Yang-Yang, Wu Wang-Yang, Gong Hui-Ling, Li Wei-Zu, Yin Yan-Yan
Department of Pharmacology, Anhui Medical University, Key Laboratory of Chinese Medicine Research and Development, State Administration of Traditional Chinese Medicine, Hefei, Anhui 230032, P.R. China.
Mol Med Rep. 2014 Apr;9(4):1319-24. doi: 10.3892/mmr.2014.1969. Epub 2014 Feb 19.
Astragalosides (ASTs) have been traditionally used in the treatment of various cardiovascular and cerebrovascular diseases. The aim of the present study was to investigate the neuroprotective effects of AST on learning and memory following focal cerebral ischemia/reperfusion in a rat model. A Morris water maze was used to measure the effect of AST on learning and memory impairments. A histological examination and Hoechst 33258 staining was used to observe the neuronal changes and apoptosis in the hippocampus. The activity of phospho-extracellular signal‑regulated kinases (p‑ERK), p‑c-Jun N-terminal kinases (JNK) and p‑Akt was measured by western blotting. The data revealed that AST improved the rats learning and memory abilities, attenuated neuronal cells apoptosis, increased the expression of p‑ERK and p‑Akt, and decreased the expression of p‑JNK. These findings indicated that AST has protective effects that may be correlated with the inhibition of neuronal cell apoptosis and the regulation of p‑ERK, p‑Akt and p‑JNK expression.
黄芪总苷（ASTs）传统上用于治疗各种心脑血管疾病。本研究的目的是在大鼠局灶性脑缺血/再灌注模型中，研究AST对学习和记忆的神经保护作用。采用Morris水迷宫来测量AST对学习和记忆损伤的影响。通过组织学检查和Hoechst 33258染色来观察海马体中的神经元变化和细胞凋亡。采用蛋白质免疫印迹法检测磷酸化细胞外信号调节激酶（p-ERK）、磷酸化c-Jun氨基末端激酶（JNK）和磷酸化Akt的活性。数据显示，AST改善了大鼠的学习和记忆能力，减轻了神经元细胞凋亡，增加了p-ERK和p-Akt的表达，并降低了p-JNK的表达。这些发现表明，AST具有保护作用，可能与抑制神经元细胞凋亡以及调节p-ERK、p-Akt和p-JNK的表达有关。
