Parekh Mohit, Salvalaio Gianni, Ferrari Stefano, Amoureux Marie-Claude, Albrecht Cecile, Fortier Denis, Ponzin Diego
International Center for Ocular Physiopathology (ICOP), The Veneto Eye Bank Foundation, Padiglione Rama-Via Paccagnella 11, 30174, Zelarino, Venice, Italy,
Cell Tissue Bank. 2014 Dec;15(4):543-54. doi: 10.1007/s10561-014-9424-2. Epub 2014 Feb 25.
To standardize a new evaluation technique for calculating the overall quality (OQ) of the donor cornea and validate it using a comparative study of corneas preserved in Optisol-GS and Cornea Cold®. Thirty pairs of donor corneas were selected for a 4 week in vitro comparative study using masked observers. Physiological parameters like thickness, transparency, viable endothelial cell density (VECD) and morphology were transformed to numerical range (0-4) to obtain the OQ. Microbiological examination was performed using Bactec instrument. Students t test showed statistically better results (p < 0.05) from week 3 for thickness, week 2 for transparency and week 1 for morphology and VECD; statistical significance (p < 0.05) was found for OQ from week 2 for the corneas preserved in Cornea Cold® compared to Optisol-GS. Epithelial quality was similar regardless of the medium. Microbiological examination showed absence of aerobic and anaerobic microorganisms in both media. OQ method is efficient, consistent and easy, now validated for comparative studies. Further refinement is necessary for its use at eye-banks, bio-banks and research or transplantation purposes. Cornea Cold® is a promising hypothermic corneal storage medium with preservation time ≤21 days. This permits higher flexibility, evaluation accuracy, longer duration for surgical preparation and ease of transportation.
为规范一种计算供体角膜总体质量（OQ）的新评估技术，并通过对保存在Optisol-GS和Cornea Cold®中的角膜进行比较研究来验证该技术。选择30对供体角膜进行为期4周的体外比较研究，由不知情的观察者进行评估。将厚度、透明度、存活内皮细胞密度（VECD）和形态等生理参数转换为数值范围（0-4）以获得OQ。使用Bactec仪器进行微生物学检查。学生t检验显示，从第3周起厚度、第2周起透明度以及第1周起形态和VECD的结果在统计学上有更好的表现（p < 0.05）；与Optisol-GS相比，在第2周时，保存在Cornea Cold®中的角膜的OQ在统计学上具有显著差异（p < 0.05）。无论使用何种培养基，上皮质量相似。微生物学检查显示两种培养基中均无需氧和厌氧微生物。OQ方法高效、一致且简便，现已在比较研究中得到验证。为了在眼库、生物库以及研究或移植目的中使用，还需要进一步完善。Cornea Cold®是一种有前景的低温角膜保存介质，保存时间≤21天。这使得其具有更高的灵活性、评估准确性、更长的手术准备时间以及便于运输。
