1型糖尿病患者血浆中对氧磷酶内酯酶活性、炎症及抗氧化状态
Paraoxonase lactonase activity, inflammation and antioxidant status in plasma of patients with type 1 diabetes mellitus.
作者信息
Savu Octavian, Serafinceanu Cristian, Grajdeanu Ioana Veronica, Iosif Liviu, Gaman Laura, Stoian Irina
机构信息
N.C. Paulescu National Institute for Diabetes, Nutrition and Metabolic Diseases, Bucharest, Romania.
出版信息
J Int Med Res. 2014 Apr;42(2):523-9. doi: 10.1177/0300060513516287. Epub 2014 Feb 24.
OBJECTIVES
To investigate paraoxonase-1 (PON1) lactonase activity, myeloperoxidase (MPO) activity (as a marker of inflammation) and antioxidant status in plasma of patients with type 1 diabetes mellitus.
METHODS
Whole blood and plasma samples were collected from patients with diabetes and healthy control subjects. PON1 lactonase and MPO activities and total antioxidant capacity (TEAC) were determined in plasma. Glycosylated haemoglobin (HbA1c) was quantified in whole blood.
RESULTS
Plasma PON1 lactonase and MPO activities were significantly higher and TEAC was significantly lower in patients with diabetes (n = 18) compared with healthy control subjects (n = 20). There were significant positive correlations between PON1 lactonase activity and MPO activity and HbA1c level, and plasma MPO and HbA1c. There were significant negative correlations between PON1 lactonase activity and TEAC, and MPO activity and TEAC.
CONCLUSIONS
Increased lactonase activity may inefficiently compensate for the high level of chronic inflammation and low antioxidant capacity in the plasma of patients with type 1 diabetes mellitus.
目的
研究1型糖尿病患者血浆中对氧磷酶-1（PON1）内酯酶活性、髓过氧化物酶（MPO）活性（作为炎症标志物）及抗氧化状态。
方法
采集糖尿病患者和健康对照者的全血及血浆样本。测定血浆中PON1内酯酶和MPO活性以及总抗氧化能力（TEAC）。对全血中的糖化血红蛋白（HbA1c）进行定量分析。
结果
与健康对照者（n = 20）相比，糖尿病患者（n = 18）血浆中PON1内酯酶和MPO活性显著升高，而TEAC显著降低。PON1内酯酶活性与MPO活性以及HbA1c水平之间，血浆MPO与HbA1c之间存在显著正相关。PON1内酯酶活性与TEAC之间，MPO活性与TEAC之间存在显著负相关。
结论
内酯酶活性增加可能无法有效补偿1型糖尿病患者血浆中高水平的慢性炎症和低抗氧化能力。
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