Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, 610041 Sichuan, China.
Laboratory of Genetic Disease and Perinatal Medicine, Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, 610041 Sichuan, China.
J Diabetes Res. 2020 Feb 3;2020:3483427. doi: 10.1155/2020/3483427. eCollection 2020.
Paraoxonase 1 (PON1) is a calcium-dependent multifunctional enzyme that binds to high-density lipoproteins. The physiological function of PON1 is related to its lactonase activity. However, this activity has not been analyzed in women with gestational diabetes mellitus (GDM). The present study investigated the lactonase activities and status of PON1 and their association with genetic variants and oxidative stress indices in Chinese women with GDM.
This is a case-control study of 347 women with GDM and 288 women with uncomplicated pregnancies. PON1 levels and lactonase activities were analyzed using 7-O-diethylphosphoryl-3-cyano-4-methyl-7-hydroxycoumarin (DEPCyMC) and 5-thiobutyl butyrolactone (TBBL), respectively. A normalized lactonase activity (NLA) was estimated based on the ratio of TBBLase to DEPCyMCase activity. Serum malondialdehyde (MDA), total oxidant status (TOS), total antioxidant capacity (TAC) levels, and genetic variants and oxidative stress indices in Chinese women with GDM.
PON1 lactonase activity and levels of TOS, TAC, and MDA were higher in the GDM women compared with the control women. The genetic variation decreased the levels and lactonase activities of PON1 in a genotype-dependent manner in the patient and control groups. GDM patients with the genotype displayed lower NLA than those with the or genotype. GDM patients with the genotype of polymorphism had significantly lower PON1 lactonase activities and NLA and tended to have decreased PON1 levels compared with those with the or genotype. Multivariable regression analysis revealed that the or variations, apolipoprotein (apo) A1, apoB, TAC, MDA, or age was significant predictors of the levels, lactonase activities, or NLA of PON1.
The lactonase activities of PON1 are increased in women with GDM. genetic variants, increased oxidative stress, and abnormalities in lipoproteins may be associated with these changes. genetic variants and oxidative stress indices in Chinese women with GDM.
对氧磷酶 1（PON1）是一种与高密度脂蛋白结合的钙依赖性多功能酶。PON1 的生理功能与其内酯酶活性有关。然而，这种活性尚未在患有妊娠糖尿病（GDM）的女性中进行分析。本研究旨在探讨中国 GDM 妇女的 PON1 内酯酶活性和状态及其与遗传变异和氧化应激指标的关系。
这是一项 347 例 GDM 妇女和 288 例非复杂妊娠妇女的病例对照研究。使用 7-O-二乙基膦酰基-3-氰基-4-甲基-7-羟基香豆素（DEPCyMC）和 5-硫代丁基丁内酯（TBBL）分别分析 PON1 水平和内酯酶活性。根据 TBBLase 与 DEPCyMCase 活性的比值估计归一化内酯酶活性（NLA）。测定中国 GDM 妇女的血清丙二醛（MDA）、总氧化剂状态（TOS）、总抗氧化能力（TAC）水平以及遗传变异和氧化应激指标。
GDM 妇女的 PON1 内酯酶活性以及 TOS、TAC 和 MDA 水平均高于对照组妇女。遗传变异以依赖于基因型的方式降低了患者和对照组中 PON1 的水平和内酯酶活性。与 或 基因型相比，携带 基因型的 GDM 患者的 NLA 较低。与 或 基因型相比，携带 多态性 基因型的 GDM 患者的 PON1 内酯酶活性和 NLA 明显降低，且 PON1 水平有降低趋势。多变量回归分析显示，或 变异、载脂蛋白（apo）A1、apoB、TAC、MDA 或年龄是 PON1 水平、内酯酶活性或 NLA 的显著预测因子。
GDM 妇女的 PON1 内酯酶活性增加。遗传变异、氧化应激增加和脂蛋白异常可能与这些变化有关。中国 GDM 妇女的遗传变异和氧化应激指标。
