通过多重稳定同位素标记对RNA修饰进行分析。
Profiling of RNA modifications by multiplexed stable isotope labelling.
作者信息
Kellner Stefanie, Neumann Jennifer, Rosenkranz David, Lebedeva Svetlana, Ketting René F, Zischler Hans, Schneider Dirk, Helm Mark
机构信息
Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, 5 Staudinger Weg, Mainz, D-55128, Germany.
出版信息
Chem Commun (Camb). 2014 Apr 4;50(26):3516-8. doi: 10.1039/c3cc49114e. Epub 2014 Feb 24.
The combination of (15)N/(13)C stable isotope labelling (SIL) and LC-MS/MS revealed a total of 52 modifications in RNA from E. coli and yeast, including 10 previously undescribed modifications. Two modifications, N-ribosylnicotinamide and 2-methylthioadenosine, were newly detected in species hitherto thought not to contain these modifications.
（15）N/（13）C稳定同位素标记（SIL）与液相色谱-串联质谱联用技术（LC-MS/MS）相结合，共揭示了大肠杆菌和酵母RNA中的52种修饰，其中包括10种此前未描述的修饰。在迄今认为不含有这些修饰的物种中，新检测到了两种修饰，即N-核糖基烟酰胺和2-甲硫基腺苷。
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