Kellner Stefanie, Neumann Jennifer, Rosenkranz David, Lebedeva Svetlana, Ketting René F, Zischler Hans, Schneider Dirk, Helm Mark
Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, 5 Staudinger Weg, Mainz, D-55128, Germany.
Chem Commun (Camb). 2014 Apr 4;50(26):3516-8. doi: 10.1039/c3cc49114e. Epub 2014 Feb 24.
The combination of (15)N/(13)C stable isotope labelling (SIL) and LC-MS/MS revealed a total of 52 modifications in RNA from E. coli and yeast, including 10 previously undescribed modifications. Two modifications, N-ribosylnicotinamide and 2-methylthioadenosine, were newly detected in species hitherto thought not to contain these modifications.
（15）N/（13）C稳定同位素标记（SIL）与液相色谱-串联质谱联用技术（LC-MS/MS）相结合，共揭示了大肠杆菌和酵母RNA中的52种修饰，其中包括10种此前未描述的修饰。在迄今认为不含有这些修饰的物种中，新检测到了两种修饰，即N-核糖基烟酰胺和2-甲硫基腺苷。
