印度米佐拉姆邦p53密码子72多态性与饮食和烟草相关习惯及胃癌风险的相互关系
p53 codon 72 polymorphism interactions with dietary and tobacco related habits and risk of stomach cancer in Mizoram, India.
作者信息
Malakar Mridul, Devi K Rekha, Phukan Rup Kumar, Kaur Tanvir, Deka Manab, Puia Lalhriat, Sailo Lalrinliana, Lalhmangaihi T, Barua Debajit, Rajguru Sanjib Kumar, Mahanta Jagadish, Narain Kanwar
机构信息
Regional Medical Research Centre, NE Region (Indian Council of Medical Research), Dibrugarh, India E-mail :
出版信息
Asian Pac J Cancer Prev. 2014;15(2):717-23. doi: 10.7314/apjcp.2014.15.2.717.
BACKGROUND
This study was carried out to investigate the interaction of p53 codon 72 polymorphism, dietary and tobacco habits with reference to risk of stomach cancer in Mizoram, India. A total of 105 histologically confirmed stomach cancer cases and 210 age, sex and ethnicity matched healthy population controls were included in this study.
MATERIALS AND METHODS
The p53 codon 72 polymorphism was detected by PCR-RFLP and sequencing. H. pylori infection status was determined by ELISA. Information on various dietary and tobacco related habits was recorded with a standard questionnaire.
RESULTS
This study revealed that overall, the Pro/ Pro genotype was significantly associated with a higher risk of stomach cancer (OR, 2.54; 95%CI, 1.01-6.40) as compared to the Arg/Arg genotype. In gender stratified analysis, the Pro/Pro genotype showed higher risk (OR, 7.50; 95%CI, 1.20-47.0) than the Arg/Arg genotype among females. Similarly, the Pro/Pro genotype demonstrated higher risk of stomach cancer (OR, 6.30; 95%CI, 1.41-28.2) among older people (>60 years). However, no such associations were observed in males and in individuals <60 years of age. Smoke dried fish and preserved meat (smoke dried/sun dried) consumers were at increased risk of stomach cancer (OR, 4.85; 95%CI, 1.91-12.3 and OR, 4.22; 95%CI, 1.46-12.2 respectively) as compared to non-consumers. Significant gene-environment interactions exist in terms of p53 codon 72 polymorphism and stomach cancer in Mizoram. Tobacco smokers with Pro/Pro and Arg/Pro genotypes were at higher risk of stomach cancer (OR, 16.2; 95%CI, 1.72-153.4 and OR, 9.45; 95%CI, 1.09-81.7 respectively) than the non-smokers Arg/Arg genotype carriers. The combination of tuibur user and Arg/Pro genotype also demonstrated an elevated risk association (OR, 4.76; 95%CI, 1.40-16.21).
CONCLUSIONS
In conclusion, this study revealed that p53 codon 72 polymorphism and dietary and tobacco habit interactions influence stomach cancer development in Mizoram, India.
背景
本研究旨在调查印度米佐拉姆邦p53密码子72多态性、饮食习惯和吸烟习惯与胃癌风险之间的相互作用。本研究共纳入105例经组织学确诊的胃癌病例和210名年龄、性别及种族匹配的健康人群作为对照。
材料与方法
采用聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）和测序检测p53密码子72多态性。采用酶联免疫吸附测定法（ELISA）确定幽门螺杆菌感染状况。通过标准问卷记录各种饮食和吸烟相关习惯的信息。
结果
本研究显示，总体而言，与Arg/Arg基因型相比，Pro/Pro基因型与胃癌风险显著相关（比值比[OR]，2.54；95%置信区间[CI]，1.01 - 6.40）。在按性别分层分析中，Pro/Pro基因型在女性中显示出比Arg/Arg基因型更高的风险（OR，7.50；95%CI，1.20 - 47.0）。同样，Pro/Pro基因型在老年人（>60岁）中显示出患胃癌的风险更高（OR，6.30；95%CI，1.41 - 28.2）。然而，在男性和年龄<60岁的个体中未观察到此类关联。与非消费者相比，食用烟熏干鱼和腌制肉（烟熏干/晒干）的消费者患胃癌的风险增加（OR分别为4.85；95%CI，1.91 - 12.3和OR，4.22；95%CI，1.46 - 12.2）。在印度米佐拉姆邦，就p53密码子72多态性与胃癌而言，存在显著的基因-环境相互作用。Pro/Pro和Arg/Pro基因型的吸烟者患胃癌的风险高于非吸烟的Arg/Arg基因型携带者（OR分别为16.2；95%CI，1.72 - 153.4和OR，9.45；95%CI，1.09 - 81.7）。嚼槟榔者与Arg/Pro基因型的组合也显示出风险升高的关联（OR, 4.76；95%CI，1.40 - 16.21）。
结论
总之，本研究表明p53密码子72多态性以及饮食和吸烟习惯的相互作用会影响印度米佐拉姆邦胃癌的发生发展。
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