Baisch H
Institute of Biophysics and Radiobiology, University of Hamburg, Federal Republic of Germany.
Cytometry. 1988 Jul;9(4):325-31. doi: 10.1002/cyto.990090409.
Three cell lines (CHO, L-929, and R1H) were investigated for their growth kinetics and the difference of exponential and quiescent state of monolayers in medium with and without serum (L-929). The noncycling populations of L-929 and R1H in medium with serum contained increased G1-phase percentages but also considerable proportions of SQ and G2Q cells. Although about 90% of the cells excluded trypan blue, the viability tested by colony assay was clearly lower than for exponentially growing cultures. CHO cells showed similar fractions of cells in G1-, S-, and G2-Q compartments but in addition considerable cell loss. The RNA content of these cells was reduced in plateau phase by 7-48% depending on cell type and residence time in the noncycling state. The data suggest that the cells suffered from nutrition depletion and were arrested in all phases of the cycle. In contrast, L-929 cells in medium without serum reduced their RNA content down to one-third that of proliferating cells and still retained the full viability as shown by the same plating efficiency in a colony assay. Since about 90% of the cells had G1 DNA content, these cells resemble true G1Q or G0 cells controlled by growth factors rather than nutritional depletion.
研究了三种细胞系（CHO、L-929和R1H）在有血清和无血清（L-929）培养基中的生长动力学以及单层细胞指数生长状态和静止状态的差异。在含血清培养基中，L-929和R1H的非循环群体中G1期百分比增加，但也含有相当比例的SQ和G2Q细胞。尽管约90%的细胞排斥台盼蓝，但通过集落试验检测的活力明显低于指数生长培养物。CHO细胞在G1、S和G2-Q区室中的细胞比例相似，但此外有相当数量的细胞损失。这些细胞的RNA含量在平台期根据细胞类型和在非循环状态下的停留时间降低了7%-48%。数据表明细胞遭受营养耗竭并在细胞周期的所有阶段停滞。相比之下，无血清培养基中的L-929细胞将其RNA含量降低至增殖细胞的三分之一，并且如集落试验中相同的接种效率所示，仍保持完全活力。由于约90%的细胞具有G1 DNA含量，这些细胞类似于由生长因子而非营养耗竭控制的真正G1Q或G0细胞。
