Frankel F R, Hsu C Y, Myers J C, Lin E, Lyttle C R, Komm B, Mohn K
Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia 19104.
DNA. 1988 Jun;7(5):347-54. doi: 10.1089/dna.1.1988.7.347.
We show that mRNAs for alpha 2(I), alpha 1(III), and alpha 2(V) collagens are regulated by estradiol in the immature rat uterus. Clones for alpha 2(I) and alpha 1(III) collagen were induced in a cDNA library prepared from estradiol-stimulated rat uterine mRNA. Alpha 2(I) collagen cDNA was identified by its characteristic hybridization pattern on Northern blots and hybridization to known probes. The sequence of alpha 1(III) collagen cDNA showed 91% amino acid sequence homology with the corresponding 525-nucleotide segment of human alpha 1(III) procollagen cDNA. Type I and type III collagen mRNAs were coordinately regulated, and showed peaks of induction at 4 and 24 hr. Three injections of estradiol, 24 hr apart, produced induction maxima 24 hr apart. The third induction, however, was suppressed relative to the first, suggesting that estrogen-stimulated factors may act to negatively regulate the uterine response to estrogen.
我们发现，在未成熟大鼠子宫中，α2（I）、α1（III）和α2（V）型胶原蛋白的信使核糖核酸（mRNAs）受雌二醇调控。从经雌二醇刺激的大鼠子宫信使核糖核酸制备的互补脱氧核糖核酸（cDNA）文库中，诱导出了α2（I）和α1（III）型胶原蛋白的克隆。α2（I）型胶原蛋白互补脱氧核糖核酸通过其在Northern印迹上的特征性杂交模式以及与已知探针的杂交得以鉴定。α1（III）型胶原蛋白互补脱氧核糖核酸的序列与人类α1（III）型前胶原蛋白互补脱氧核糖核酸相应的525个核苷酸片段显示出91%的氨基酸序列同源性。I型和III型胶原蛋白信使核糖核酸受到协同调控，并在4小时和24小时出现诱导峰值。每隔24小时注射三次雌二醇，会相隔24小时产生诱导最大值。然而，第三次诱导相对于第一次受到抑制，这表明雌激素刺激的因子可能起到负调控子宫对雌激素反应的作用。
