Hsu C Y, Frankel F R
J Biol Chem. 1987 Jul 15;262(20):9594-600.
Cytoplasmic beta- and gamma-actin mRNAs as well as smooth muscle actin mRNAs have been shown to be transiently increased in rat uterus after treatment with the steroid hormone estradiol. A clone isolated as an estradiol-induced message from a lambda-gt10 cDNA library prepared from the mRNA of estrogen-stimulated immature rat uterus was identified as alpha-smooth muscle actin. A single-stranded RNA probe composed mainly of the 3'-untranslated region of this clone, as well as DNA probes derived from the 3'-untranslated regions of other actin genes, were used to study the induction kinetics of different actin isoforms in rat uterus after being stimulated by estradiol. The beta- and gamma-cytoskeletal actins showed an induction peak at 4 h after estradiol administration with 1.4- and 1.8-fold increases, respectively. The smooth muscle actin was maximally increased 2.1-fold at 8-12 h. Messages of alpha-skeletal and alpha-cardiac actins were neither expressed nor induced by estradiol in this tissue. The different induction kinetics of the cytoplasmic and smooth muscle actins suggest that they are regulated by different mechanisms and possibly in different cell types of the uterus.
已表明，在用甾体激素雌二醇处理后，大鼠子宫中的细胞质β-肌动蛋白和γ-肌动蛋白mRNA以及平滑肌肌动蛋白mRNA会短暂增加。从雌激素刺激的未成熟大鼠子宫mRNA制备的λ-gt10 cDNA文库中分离出的一个作为雌二醇诱导信息的克隆被鉴定为α-平滑肌肌动蛋白。使用主要由该克隆的3'-非翻译区组成的单链RNA探针以及源自其他肌动蛋白基因3'-非翻译区的DNA探针，研究了雌二醇刺激后大鼠子宫中不同肌动蛋白异构体的诱导动力学。β-和γ-细胞骨架肌动蛋白在给予雌二醇后4小时出现诱导峰值，分别增加1.4倍和1.8倍。平滑肌肌动蛋白在8 - 12小时时最大增加2.1倍。α-骨骼肌肌动蛋白和α-心肌肌动蛋白的信息在该组织中既未表达也未被雌二醇诱导。细胞质肌动蛋白和平滑肌肌动蛋白不同的诱导动力学表明它们受不同机制调节，可能在子宫的不同细胞类型中发挥作用。
