McAteer M J, Lagarde A C, Georgiou H M, Bellgrau D
Barbara Davis Center for Childhood Diabetes, Department of Microbiology and Immunology, University of Colorado Health Sciences Center, Denver 80262.
Eur J Immunol. 1988 Jul;18(7):1111-7. doi: 10.1002/eji.1830180721.
Treatment of adult rats with a monoclonal antibody specific for the CD5 antigen led to a dramatic reduction in the number of CD5+ cells. However, a substantial number of T cells remained as assessed by other T cell-specific antibodies. These CD5- T cells did not proliferate in response to alloantigen or mitogenic stimulation, did not generate cytotoxic T lymphocytes in vitro, and did not induce graft-vs.-host disease when injected into susceptible recipients in vivo. Re-expression of the CD5 antigen occurred when CD5- T cells were placed in an environment devoid of the anti-CD5 antibody. Re-expression of the antigen was followed by return of the T cell proliferative responses. While CD5- T cells could not proliferate in response to alloantigen they could produce interleukin 2 following a short pulse with the T cell mitogen concanavalin A. However, T cell proliferative or cytotoxic responses could not be rescued by the addition of an exogenous source of interleukin 2. We conclude that the CD5 antigen appears to be required for proliferation of resting T cells.
用针对CD5抗原的单克隆抗体处理成年大鼠，导致CD5+细胞数量急剧减少。然而，通过其他T细胞特异性抗体评估，仍有相当数量的T细胞存在。这些CD5-T细胞在同种异体抗原或促有丝分裂刺激下不增殖，在体外不产生细胞毒性T淋巴细胞，并且在体内注射到易感受体中时不诱导移植物抗宿主病。当将CD5-T细胞置于不含抗CD5抗体的环境中时，CD5抗原会重新表达。抗原重新表达后，T细胞增殖反应恢复。虽然CD5-T细胞不能对同种异体抗原增殖，但在与T细胞促有丝分裂原伴刀豆球蛋白A短暂接触后，它们可以产生白细胞介素2。然而，添加外源性白细胞介素2并不能挽救T细胞增殖或细胞毒性反应。我们得出结论，静止T细胞的增殖似乎需要CD5抗原。
