一种用于钒的计时电流法测定的基于一次性碱性磷酸酶的生物传感器。

CELEQ and School of Chemistry, University of Costa Rica, San Pedro de Montes de Oca, San José P.O. Box 11500-2060, Costa Rica.

Department of Chemistry, Faculty of Sciences, University of Burgos, Plaza Misael Bañuelos s/n, Burgos 09001, Spain.

Sensors (Basel). 2014 Feb 24;14(2):3756-67. doi: 10.3390/s140203756.

A chronoamperometric method for vanadium ion determination, based on the inhibition of the enzyme alkaline phosphatase, is reported. Screen-printed carbon electrodes modified with gold nanoparticles were used as transducers for the immobilization of the enzyme. The enzymatic activity over 4-nitrophenyl phosphate sodium salt is affected by vanadium ions, which results in a decrease in the chronoamperometric current registered. The developed method has a detection limit of 0.39 ± 0.06 µM, a repeatability of 7.7% (n = 4) and a reproducibility of 8% (n = 3). A study of the possible interferences shows that the presence of Mo(VI), Cr(III), Ca(II) and W(VI), may affect vanadium determination at concentration higher than 1.0 mM. The method was successfully applied to the determination of vanadium in spiked tap water.

本文报道了一种基于抑制酶碱性磷酸酶的伏安计时法来测定钒离子的方法。采用金纳米粒子修饰的丝网印刷碳电极作为酶的固定化传感器。钒离子会影响对 4-硝基苯磷酸二钠盐的酶活性，从而导致计时电流的减少。该方法的检测限为 0.39 ± 0.06 µM，重复性为 7.7%（n = 4），重现性为 8%（n = 3）。对可能存在的干扰进行研究表明，当钼(VI)、铬(III)、钙(II)和钨(VI)的浓度高于 1.0 mM 时，可能会影响钒的测定。该方法成功应用于加标自来水中钒的测定。