分泌脂多糖A反应性单克隆抗体的人-人杂交瘤细胞

Human--human hybridomas secreting lipid A reactive monoclonal antibodies.

作者信息

Ramachandra R N, Berczi I, Sehon A H

机构信息

Department of Immunology, University of Manitoba, Winnipeg, Canada.

出版信息

Immunol Lett. 1988 Jun;18(2):93-7. doi: 10.1016/0165-2478(88)90047-8.

DOI:10.1016/0165-2478(88)90047-8

PMID:2456987

Abstract

Five human-human hybridoma cell lines secreting monoclonal antibodies (mAbs) against lipid A (LA) were produced by cell fusion of Epstein-Barr virus (EBV) transformed human peripheral blood lymphocytes and a human lymphoblastoid cell line KR-4. All these mAbs were isotyped as IgM(kappa) and reacted with lipopolysaccharides (LPS) and LA of various gram-negative bacteria. Whereas the binding of only four of the five mAbs to solid-phase LA was blocked by polymyxin-B sulphate, the mitogenic effect of LPS and LA on murine B lymphocytes was inhibited by all five mAbs. These results demonstrate that the human immune system recognizes at least two common epitopes in lipid A of various gram-negative bacteria.

摘要

通过爱泼斯坦 - 巴尔病毒（EBV）转化的人外周血淋巴细胞与人类淋巴母细胞系KR - 4的细胞融合，产生了五种分泌抗脂质A（LA）单克隆抗体（mAb）的人 - 人杂交瘤细胞系。所有这些单克隆抗体的免疫球蛋白类型均为IgM（κ），并与各种革兰氏阴性菌的脂多糖（LPS）和脂质A发生反应。虽然五种单克隆抗体中只有四种与固相脂质A的结合被硫酸多粘菌素B阻断，但所有五种单克隆抗体均抑制了LPS和脂质A对小鼠B淋巴细胞的促有丝分裂作用。这些结果表明，人类免疫系统识别各种革兰氏阴性菌脂质A中至少两个共同表位。