分泌抗乙型肝炎病毒表面抗原人单克隆抗体杂交瘤的建立。
Establishment of hybridoma secreting human monoclonal antibody against hepatitis B virus surface antigen.
作者信息
Ichimori Y, Harada K, Hitotsumachi S, Tsukamoto K
出版信息
Biochem Biophys Res Commun. 1987 Feb 13;142(3):805-12. doi: 10.1016/0006-291x(87)91485-9.
The HAT (hypoxanthine, aminopterin, thymidine) sensitive and ouabain resistant human B lymphoblastoid cell line TAW-925 was obtained from 6-thioguanine resistant B lymphoblastoid cell line WI-L2. Hybridomas were obtained at a high frequency (10(-4)-10(-5) when TAW-925 was hybridized with cells transformed with Epstein-Barr virus. Using TAW-925 as a parental cell line, we have obtained a hybridoma which stably secretes human monoclonal antibody against hepatitis B virus surface antigen.
对次黄嘌呤、氨基蝶呤、胸腺嘧啶核苷（HAT）敏感且对哇巴因耐药的人B淋巴母细胞系TAW-925是从对6-硫鸟嘌呤耐药的B淋巴母细胞系WI-L2获得的。当TAW-925与经爱泼斯坦-巴尔病毒转化的细胞杂交时，能以高频率（10⁻⁴ - 10⁻⁵）获得杂交瘤。以TAW-925作为亲本细胞系，我们获得了一种能稳定分泌抗乙型肝炎病毒表面抗原人单克隆抗体的杂交瘤。
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