Nicholson L J, Jani H
Department of Oncology, Middlesex Hospital Medical School, London, UK.
Int J Cancer. 1988 Aug 15;42(2):295-8. doi: 10.1002/ijc.2910420226.
The tumour-promoting activities of sodium cyclamate and sodium saccharin were investigated in an assay based on the induction of epithelial foci exhibiting enhanced growth potential in a rat bladder explant culture system. An initiating, non-focus-inducing dose was defined for the carcinogen N-methyl-N-nitrosourea (MNU) to make promotion studies possible. Saccharin induced epithelial foci when added to cultures pretreated with an initiating dose of MNU, and also increased the incidence of foci in cultures treated with transforming doses of MNU. Cyclamate was found to induce a high incidence of foci when added to cultures by itself. When MNU and cyclamate treatments were combined, an additive effect could be detected. These results indicate that both cyclamate and saccharin can contribute to epithelial transformation in this system.
在基于大鼠膀胱外植体培养系统中诱导具有增强生长潜力的上皮灶的实验中，研究了甜蜜素钠和糖精钠的促肿瘤活性。为致癌物N-甲基-N-亚硝基脲（MNU）确定了引发但不诱导灶形成的剂量，以便进行促癌研究。当将糖精添加到用引发剂量的MNU预处理的培养物中时，可诱导上皮灶形成，并且还增加了用转化剂量的MNU处理的培养物中灶的发生率。发现甜蜜素单独添加到培养物中时可诱导高发生率的灶形成。当联合使用MNU和甜蜜素处理时，可检测到相加效应。这些结果表明，甜蜜素和糖精在该系统中均可促进上皮转化。
