白色念珠菌生物膜形成与无脊椎动物宿主大蜡螟侵染之间的相关性

Correlation between Candida albicans biofilm formation and invasion of the invertebrate host Galleria mellonella.

作者信息

Borghi Elisa, Romagnoli Solange, Fuchs Beth Burgwyn, Cirasola Daniela, Perdoni Federica, Tosi Delfina, Braidotti Paola, Bulfamante Gaetano, Morace Giulia, Mylonakis Eleftherios

机构信息

Department of Health Sciences, Università degli Studi di Milano, via di Rudinì, 8 - 20142 Milan, Italy.

出版信息

Future Microbiol. 2014;9(2):163-73. doi: 10.2217/fmb.13.159.

DOI:10.2217/fmb.13.159

PMID:24571071

Abstract

AIM

The aim of our study was to investigate whether biofilm production by Candida albicans clinical isolates could be a hallmark of virulence in vivo.

MATERIALS & METHODS: Twenty clinical isolates of C. albicans were examined via histological studies on larvae infected with various fungal doses (from 10(3) to 10(5) CFU/larva) of biofilm producer and nonproducer strains.

RESULTS

The poor prognostic role of infection due to a biofilm-producing isolate was confirmed by the Wald test (hazard ratio: 2.63; 95% CI: 2.03-3.41). Histological examinations at 24 h showed a strong innate immune response, with evidence of melanization for both infection groups. However, at 48 h, we found huge differences in filamentation and tissue invasion capability between biofilm nonproducing and producing isolates, the latter being highly organized into biofilm and invading the larval intestinal tract. Invasion corroborated survival data.

CONCLUSION

The histological results demonstrate that the production of biofilm could enhance the invasiveness of C. albicans.

摘要

目的

我们研究的目的是调查白色念珠菌临床分离株产生生物膜是否可能是体内毒力的一个标志。

材料与方法

通过对感染不同真菌剂量（从10³到10⁵CFU/幼虫）的生物膜产生菌和非产生菌菌株的幼虫进行组织学研究，检查了20株白色念珠菌临床分离株。

结果

Wald检验证实了由产生生物膜的分离株引起的感染的不良预后作用（风险比：2.63；95%置信区间：2.03 - 3.41）。24小时的组织学检查显示出强烈的先天免疫反应，两个感染组均有黑化迹象。然而，在48小时时，我们发现生物膜非产生菌和产生菌分离株在丝状化和组织侵袭能力方面存在巨大差异，后者高度组织形成生物膜并侵入幼虫肠道。侵袭情况与生存数据相符。