Haseloff J, Gerlach W L
CSIRO Division of Plant Industry, Canberra, Australia.
Nature. 1988 Aug 18;334(6183):585-91. doi: 10.1038/334585a0.
In vitro mutagenesis of sequences required for the self-catalysed cleavage of a plant virus satellite RNA has allowed definition of an RNA segment with endoribonuclease activity. General rules have been deduced for the design of new RNA enzymes capable of highly specific RNA cleavage, and have been successfully tested against a new target sequence.
对植物病毒卫星RNA自我催化切割所需序列进行体外诱变，已确定了一个具有核糖核酸内切酶活性的RNA片段。已推导出用于设计能够进行高度特异性RNA切割的新型RNA酶的一般规则，并已针对新的靶序列成功进行了测试。
