Schwab R, Weksler M E, Russo C
Department of Medicine, Cornell University Medical College, New York, New York 10021.
Cell Immunol. 1988 Sep;115(2):310-24. doi: 10.1016/0008-8749(88)90184-0.
The role of distinct regions of HLA class I molecules in regulating T-cell activation via the CD3-antigen receptor complex was investigated. Monoclonal antibodies (MoAbs) which recognize monomorphic and polymorphic epitopes on HLA Class I molecules were shown to inhibit T-cell proliferation to OKT3. These MoAbs have differential effects on the synthesis of interleukin-2 (IL-2) and IL-2 receptor expression. Cell cycle analysis demonstrated that these MoAbs function both in inhibiting cell cycle entry (G0-G1 shift) and in blocking cell cycle progression (G1-S shift) of activated T cells. Furthermore, these MoAbs have regulatory effects on the alternate pathway of T-cell activation via the CD2 molecule, T-cell activation induced by PHA, and activation induced by the phorbol ester PMA in conjunction with the calcium ionophore Ionomycin. Thus these MoAbs have different effects depending upon the pathway of T-cell activation. The results indicate that HLA class I molecules are selectively involved in the sequence of intracellular events leading to T-cell activation and proliferation.
研究了HLA I类分子不同区域通过CD3抗原受体复合物调节T细胞活化的作用。已证明识别HLA I类分子上单一型和多态型表位的单克隆抗体（MoAbs）可抑制T细胞对OKT3的增殖反应。这些MoAbs对白细胞介素-2（IL-2）的合成和IL-2受体表达有不同影响。细胞周期分析表明，这些MoAbs在抑制活化T细胞进入细胞周期（G0-G1期转换）和阻断细胞周期进程（G1-S期转换）方面均发挥作用。此外，这些MoAbs对通过CD2分子的T细胞活化替代途径、PHA诱导的T细胞活化以及佛波酯PMA与钙离子载体离子霉素联合诱导的活化均有调节作用。因此，这些MoAbs根据T细胞活化途径产生不同影响。结果表明，HLA I类分子选择性地参与导致T细胞活化和增殖的细胞内事件序列。
