肝提取物对淋巴细胞增殖的抑制作用机制：II. 对DNA、RNA和蛋白质合成的抑制及其与代谢抑制剂作用的关系。
The mechanisms of inhibitory effects of liver extract on lymphocyte proliferation: II. Inhibition of DNA, RNA, and protein synthesis and their relationship to the effects of metabolic inhibitors.
作者信息
Su H L, Huang M H, Yu C L, Han S H, Chiang B N, Wang S R
机构信息
Department of Medicine, Veterans General Hospital, Taipei, Taiwan, ROC.
出版信息
Clin Exp Immunol. 1988 May;72(2):228-32.
The inhibitory effects of liver extract (LEx) on DNA, RNA, and protein synthesis in phytohaemagglutinin (PHA)-stimulated lymphocytes were studied by measuring the incorporation of labelled precursors. DNA, RNA, and protein synthesis were all inhibited by LEx in a dose-related manner. The inhibitory effect of LEx on protein synthesis was additive to the inhibitory effect of puromycin. The inhibitory effect of LEx on DNA synthesis was antagonistic to the inhibitory effect of mitomycin C. In the case of actinomycin D for RNA synthesis, its interactions with LEx were variable, but not additive. LEx may act as an inhibitor of protein synthesis through the mechanism of arginine-depletion.
通过测量标记前体的掺入，研究了肝提取物（LEx）对植物血凝素（PHA）刺激的淋巴细胞中DNA、RNA和蛋白质合成的抑制作用。LEx对DNA、RNA和蛋白质合成均有剂量依赖性抑制作用。LEx对蛋白质合成的抑制作用与嘌呤霉素的抑制作用相加。LEx对DNA合成的抑制作用与丝裂霉素C的抑制作用拮抗。对于放线菌素D对RNA合成的作用，其与LEx的相互作用是可变的，但不是相加的。LEx可能通过精氨酸耗竭机制作为蛋白质合成的抑制剂。
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