印度卡纳塔克邦水稻（Oryza sativa L.）上产毒层出镰刀菌的自然发生情况。
Natural Occurrence of Toxigenic Fusarium proliferatum on Paddy (Oryza sativa L.) in Karnataka, India.
作者信息
Maheshwar Pavagada Krishnamurthy, Janardhana Gottravalli Ramanayaka
机构信息
Department of Microbiology, Yuvaraja's College, University of Mysore, Mysore 570 005, Karnataka, India.
Department of Studies in Botany, University of Mysore, Manasagangotri, Mysore 570 006, Karnataka, India.
出版信息
Trop Life Sci Res. 2010 Aug;21(1):1-10.
Contamination of paddy seeds (rice with husk) by Fusarium species can cause spoilage and subsequent production of mycotoxins, especially fumonisins that affect human and animal health. A mycological study was conducted to evaluate the natural occurrence of fumonisin B1 produced by Fusarium proliferatum on paddy grown in different geographic regions of Karnataka (India). A total of 65 isolates of F. proliferatum from paddy samples were analysed by polymerase chain reaction (PCR). One set of primers, Fp3-F and Fp4-R was employed to identify the species F. proliferatum, and another set of primers, FUM1 was employed to determine the fumonisin producing ability of the isolates. All 65 isolates of F. proliferatum scored positive with both set of primers, producing amplified products of the expected sizes. Furthermore, thin layer chromatography (TLC) analysis detected fumonisin B1 (FB1) in all of the PCR positive isolates of F. proliferatum.
镰刀菌属对稻谷种子（带壳水稻）的污染会导致种子变质并随后产生霉菌毒素，尤其是影响人类和动物健康的伏马毒素。开展了一项真菌学研究，以评估在印度卡纳塔克邦不同地理区域种植的稻谷上，轮枝镰刀菌产生伏马毒素B1的自然情况。通过聚合酶链反应（PCR）对从稻谷样品中分离出的总共65株轮枝镰刀菌进行了分析。一组引物Fp3-F和Fp4-R用于鉴定轮枝镰刀菌物种，另一组引物FUM1用于确定分离株产生伏马毒素的能力。所有65株轮枝镰刀菌分离株在两组引物检测中均呈阳性，产生预期大小的扩增产物。此外，薄层色谱（TLC）分析在所有PCR检测呈阳性的轮枝镰刀菌分离株中均检测到了伏马毒素B1（FB1）。
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