Bragulat M R, Martínez E, Castellá G, Cabañes F J
Departament de Sanitat i d'Anatomia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, E-08193 Bellaterra, Barcelona, Spain.
J Food Prot. 2004 Jan;67(1):207-11. doi: 10.4315/0362-028x-67.1.207.
Selective culture media, such as Nash and Snyder medium (NS), dichloran-chloramphenicol peptone agar (DCPA), modified Czapek-Dox agar (MCz), Czapek Dox iprodione dichloran agar (CZID), potato dextrose iprodione dichloran agar (PDID), or malachite green agar (MGA 2.5), have been developed for isolating and enumerating Fusarium spp. from natural samples. However, some of these culture media are not very selective because they allow the growth of many other fungal species. In this study, a comparison of the selective efficacy of these culture media, using different strains of Fusarium spp. (F. anthophilum, F. culmorum, F. dlamini, F. graminearum, F. napiforme, F. nygamai, F. oxysporum, F. proliferatum, F. semitectum, F. solani, F. subglutinans, and F. verticillioides) and natural samples has been carried out. Among the six recommended selective culture media assayed, no statistical differences were detected in colony counts of the Fusarium spp. strains tested, although the colony diameters in MGA 2.5 were significantly lower than in NS, MCz, DCPA, CZID, and PDID media. With natural samples, MGA 2.5 performs as a potent selective medium for Fusarium spp., whereas the other recommended selective media allow the growth of many other different fungal species including Zygomycetes and yeasts.
选择性培养基，如纳什和斯奈德培养基（NS）、二氯苯胺-氯霉素蛋白胨琼脂（DCPA）、改良察氏琼脂（MCz）、察氏-异菌脲-二氯苯胺琼脂（CZID）、马铃薯葡萄糖-异菌脲-二氯苯胺琼脂（PDID）或孔雀石绿琼脂（MGA 2.5），已被开发用于从自然样品中分离和计数镰刀菌属。然而，这些培养基中的一些选择性不强，因为它们允许许多其他真菌物种生长。在本研究中，使用不同的镰刀菌属菌株（嗜花镰刀菌、禾谷镰刀菌、德氏镰刀菌、禾谷镰刀菌、芜菁镰刀菌、奈加马镰刀菌、尖孢镰刀菌、轮枝镰刀菌、半裸镰刀菌、茄病镰刀菌、亚粘团镰刀菌和串珠镰刀菌）和自然样品，对这些培养基的选择效果进行了比较。在所检测的六种推荐选择性培养基中，尽管MGA 2.5中的菌落直径显著低于NS、MCz、DCPA、CZID和PDID培养基，但在所测试的镰刀菌属菌株的菌落计数中未检测到统计学差异。对于自然样品，MGA 2.5是一种有效的镰刀菌属选择性培养基，而其他推荐的选择性培养基允许许多其他不同的真菌物种生长，包括接合菌和酵母。
