Mohiuddin Abul Kashem Md, Karim Nilufer Hye, Sultana Shahanaz, Ferdous Zannatul
Department of Biotechnology and Genetic Engineering, Mawlana Bhashani Science and Technology University, Santosh, Tangail 1902, Bangladesh.
Biotechnology Division, Bangladesh Rice Research Institute, Gazipur 1701, Bangladesh.
Trop Life Sci Res. 2011 May;22(1):1-12.
A simple method was developed to permit albino plant regeneration from anther culture of Hobigonj Boro (Hbj B) IV and Hbj B VI, two local varieties of aromatic indica rice from Bangladesh. Three crucial factors were identified for the albino shoot primordia to change into green plantlets in culture; components of M10 induction medium, callus size (range 0.2-0.4 cm long) and height of shoot primordia (range 2-3 mm). Immediate transfer of shoot primordia (2-3 mm) from M10 medium to regeneration medium followed by continuous incubation under fluorescent light (100-lux, 25±1°C) triggered albino shoot primordia to turn green in 2-3 days. Callus size did not show any effect on the change. Albino plantlets derived from anther callus cultured in KA, KB, KC, KD and KE media did not recover in both the varieties. Transfer of albino shoot primordia shorter or longer than 2-3 mm from the above 5 cultures to regeneration medium did not cause the shoot primordia to turn green. 100% albino shoot primordia initiated from Hbj B VI and 79% from Hbj B IV in M10 medium changed to green plantlets upon transfer to regeneration medium. Subsequent culture and subculture of green plantlets showed rapid formation of many new green plantlets.
开发了一种简单的方法，可从孟加拉国的两个地方香稻品种霍比贡杰博洛（Hbj B）IV和Hbj B VI的花药培养物中再生白化苗。确定了三个关键因素，以使白化苗原基在培养中转变为绿色小植株；M10诱导培养基的成分、愈伤组织大小（范围为0.2 - 0.4厘米长）和苗原基高度（范围为2 - 3毫米）。将苗原基（2 - 3毫米）从M10培养基立即转移到再生培养基，然后在荧光灯下（100勒克斯，25±1°C）连续培养，可使白化苗原基在2 - 3天内变绿。愈伤组织大小对这种转变没有任何影响。在KA、KB、KC、KD和KE培养基中培养的花药愈伤组织产生的白化苗在这两个品种中都没有恢复。将上述5种培养物中短于或长于2 - 3毫米的白化苗原基转移到再生培养基中，不会使苗原基变绿。在M10培养基中，从Hbj B VI起始的100%白化苗原基和从Hbj B IV起始的79%白化苗原基转移到再生培养基后转变为绿色小植株。随后对绿色小植株进行继代培养，显示出快速形成许多新的绿色小植株。
