Tokunaga T, Yamamoto S, Namba K
Department of Cellular Immunology, National Institute of Health, Tokyo.
Jpn J Cancer Res. 1988 Jun;79(6):682-6. doi: 10.1111/j.1349-7006.1988.tb02222.x.
When various synthetic double- or single-stranded DNAs were incubated with spleen cells from mice (BALB/c or CDF1) at 37 degrees for 20 hr, it was found that some of the DNAs augmented NK activity and produced factors in the culture supernatants which showed antiviral activity and activity to render mouse macrophages cytotoxic toward tumor cells. Poly(dG,dC) showed the strongest activities, when incubated with spleen cells from lipopolysaccharide-nonresponsive mice, C3H/HeJ. The activity of the culture supernatant to activate macrophages was completely abolished by a small amount of anti-IFN gamma antibody. On the other hand, the virus-inhibitory activity of the supernatant was mostly neutralized by anti-IFN alpha/beta. When IMC tumor cells (5 x 10(5) cells) were mixed with poly(dG,dC) (100 micrograms) and then inoculated intradermally into CDF1 mice, the tumor did not take, while tumors grew progressively and killed the mice in a control group inoculated with tumor cells alone. Direct cytotoxicity of poly(dG,dC) at a concentration of 1,000 micrograms/ml against IMC cells was not observed in vitro.
当各种合成的双链或单链DNA与小鼠（BALB/c或CDF1）的脾细胞在37℃孵育20小时时，发现一些DNA增强了自然杀伤（NK）活性，并在培养上清液中产生了具有抗病毒活性以及使小鼠巨噬细胞对肿瘤细胞产生细胞毒性活性的因子。当与来自脂多糖无反应小鼠C3H/HeJ的脾细胞一起孵育时，聚（dG，dC）表现出最强的活性。培养上清液激活巨噬细胞的活性被少量抗干扰素γ抗体完全消除。另一方面，上清液的病毒抑制活性大部分被抗干扰素α/β中和。当将IMC肿瘤细胞（5×10⁵个细胞）与聚（dG，dC）（100微克）混合，然后皮内接种到CDF1小鼠体内时，肿瘤未形成，而在仅接种肿瘤细胞的对照组中，肿瘤逐渐生长并导致小鼠死亡。在体外未观察到浓度为1000微克/毫升的聚（dG，dC）对IMC细胞的直接细胞毒性。
