Yamamoto S, Kuramoto E, Shimada S, Tokunaga T
Department of Cellular Immunology, National Institute of Health, Tokyo.
Jpn J Cancer Res. 1988 Jul;79(7):866-73. doi: 10.1111/j.1349-7006.1988.tb00049.x.
A nucleic acid-rich fraction extracted and purified from BCG (MY-1) augmented natural killer (NK) cell activity of mouse spleen cells in vitro, and produced factor(s) which showed anti-viral activity and rendered normal macrophages cytotoxic towards tumor cells. These cellular responses were induced by the MY-1 digested preliminarily with RNase, but not by the MY-1 digested with DNase, indicating that DNA contained in MY-1 was essential for the responses. The function of the factor to activate macrophages was destroyed by treatment with a small amount of anti-interferon (IFN)-gamma antiserum or under acidic conditions (pH 2), but not by treatment with anti-IFN-alpha/beta antiserum, while the anti-viral activity was destroyed almost completely by treatment with anti-IFN-alpha/beta antiserum. It appears that DNA from BCG stimulated mouse spleen cells in vitro, resulting in augmentation of NK activity and production of IFN-alpha/beta and -gamma.
从卡介苗（MY-1）中提取并纯化的富含核酸的组分，在体外增强了小鼠脾细胞的自然杀伤（NK）细胞活性，并产生了具有抗病毒活性的因子，使正常巨噬细胞对肿瘤细胞具有细胞毒性。这些细胞反应是由先用核糖核酸酶预处理的MY-1诱导的，而不是由用脱氧核糖核酸酶处理的MY-1诱导的，这表明MY-1中所含的DNA对这些反应至关重要。该因子激活巨噬细胞的功能在用少量抗干扰素（IFN）-γ抗血清处理或在酸性条件（pH 2）下被破坏，但用抗IFN-α/β抗血清处理则不会，而抗病毒活性在用抗IFN-α/β抗血清处理后几乎完全被破坏。似乎卡介苗的DNA在体外刺激小鼠脾细胞，导致NK活性增强以及IFN-α/β和 -γ的产生。
