School of Chemistry and Chemical Engineering, Southeast University , Jiangning District, Nanjing 211189, P.R. China.
Anal Chem. 2014 Apr 1;86(7):3602-9. doi: 10.1021/ac500173d. Epub 2014 Mar 13.
MicroRNAs (miRNAs) has been identified as diagnostic and prognostic biomarkers and predictors of drug response for many diseases, including a broad range of cancers, heart disease, and neurological diseases. The noninvasive theranostics system for miRNAs is very important for diagnosis and therapy of the cellular disease. Herein, a target-cell-specific theranostics nanoprobe for target-cell-specific delivery, cancer cells and intracellular miRNA-21 imaging, and cancer cell growth inhibition was proposed. The nanoprobe (FS-AS/MB) was prepared by simultaneously coupling of the AS1411 aptamer and miRNA-21 molecular beacon (miR-21-MB) onto the surface of Ru(bpy)₃²⁺-encapsulated silica (FS) nanoparticles. The FS nanoparticles synthesized by a facile reverse microemulsion method showed nearly monodisperse spherical shape with a smooth surface, good colloidal stability, a fluorescence quantum yield of ~21%, and low cytotoxicity. The antibiofouling polymer PEG grafted onto a silica shell reduced nonspecific uptake of cells. The ability of FS-AS/MB for target-specific cells delivery, simultaneous cancer cells, intracellular miRNA-21 imaging, and inhibition of miRNA-21 function and suppression of cell growth in vitro, were also demonstrated. The results of the present study suggested that the proposed nanoprobes would be a promising theranostics for different cancers by imaging and inhibiting other intracellular genes.
微小 RNA（miRNA）已被确定为许多疾病的诊断和预后生物标志物以及药物反应的预测因子，包括广泛的癌症、心脏病和神经疾病。用于 miRNA 的非侵入性治疗诊断系统对于细胞疾病的诊断和治疗非常重要。在此，提出了一种用于靶细胞特异性递药、癌细胞和细胞内 miRNA-21 成像以及癌细胞生长抑制的靶细胞特异性治疗诊断纳米探针。该纳米探针（FS-AS/MB）是通过将 AS1411 适体和 miRNA-21 分子信标（miR-21-MB）同时偶联到包裹 Ru(bpy)₃²⁺的二氧化硅（FS）纳米颗粒表面上而制备的。通过简便的反相微乳液法合成的 FS 纳米颗粒具有近单分散的球形形状、光滑的表面、良好的胶体稳定性、约 21%的荧光量子产率和低细胞毒性。接枝在二氧化硅壳上的抗生物污染聚合物 PEG 减少了细胞的非特异性摄取。还证明了 FS-AS/MB 用于靶特异性细胞递药、同时进行癌细胞、细胞内 miRNA-21 成像以及抑制 miRNA-21 功能和体外抑制细胞生长的能力。本研究的结果表明，所提出的纳米探针通过成像和抑制其他细胞内基因，将成为不同癌症的有前途的治疗诊断方法。
