Luo Yun-Ling, Huang Xin-Chun, Tu Wei-Ming, Hsu Hsin-Yun
Department of Applied Chemistry, National Chiao-Tung University, No.1001 Ta-Hsueh Road, Hsinchu 30010, Taiwan, ROC.
Department of Applied Chemistry, National Chiao-Tung University, No.1001 Ta-Hsueh Road, Hsinchu 30010, Taiwan, ROC; Institute of Molecular Science, National Chiao-Tung University, No.1001 Ta-Hsueh Road, Hsinchu 30010, Taiwan, ROC.
Anal Chim Acta. 2016 Jan 1;902:196-204. doi: 10.1016/j.aca.2015.11.018. Epub 2015 Nov 26.
We synthesized a biothiol-sensitive nanoprobe by assembling gold nanoparticles with a novel redox-responsive silica (ReSi) matrix using dithiobis (succinimidyl propionate) and (3-aminopropyl) trimethoxysilane. Thin layer disulfide-bonded networks of the ReSi could differentially respond to extra- and intracellular glutathione in cancer cells within 30 min; furthermore, targeted cellular uptake could be monitored in situ by fluorescence recovery. Sigmoidal dose-response pattern of the nanoprobes presented in this study were attributed to the buried disulfide-linked 3D nanostructure of the ReSi nanoshell, optimized at an appropriate thickness, enabling not only buffering of small redox disturbances in the extracellular milieu but also the satisfied sensitivity for rapid redox sensing. Such a ReSi-functionalized gold nanoparticle-based nanoconjugate possesses the potential to serve as an effective intracellular drug carrier for future cancer theranostics.
我们通过使用二硫代双（琥珀酰亚胺丙酸酯）和（3-氨丙基）三甲氧基硅烷，将金纳米颗粒与新型氧化还原响应性二氧化硅（ReSi）基质组装，合成了一种生物硫醇敏感的纳米探针。ReSi的薄层二硫键连接网络能够在30分钟内对癌细胞内的细胞外和细胞内谷胱甘肽产生不同的响应；此外，可通过荧光恢复原位监测靶向细胞摄取。本研究中呈现的纳米探针的S形剂量响应模式归因于ReSi纳米壳的埋藏二硫键连接的三维纳米结构，其厚度经过优化，不仅能够缓冲细胞外环境中的小氧化还原干扰，还能实现快速氧化还原传感的满意灵敏度。这种基于ReSi功能化金纳米颗粒的纳米共轭物具有作为未来癌症治疗诊断有效细胞内药物载体的潜力。
