Department of Gynecology and Obstetrics, Wenzhou Medical University Affiliated No. 1 Hospital, Wenzhou, PR China.
Gynecol Obstet Invest. 2014;77(3):150-5. doi: 10.1159/000358831. Epub 2014 Feb 27.
BACKGROUND/AIMS: The endothelial nitric oxide synthase (eNOS) gene has been enlisted by previous research as a candidate gene of preeclampsia predisposition. This study investigates the specific roles of 3 polymorphisms of the eNOS gene in a population of Chinese origin from mainland China.
We studied the association of 3 commonly studied polymorphisms of the eNOS gene, namely 4b/a, T-786C and Glu298Asp, in a case-controlled sample of 220 patients diagnosed with preeclampsia and 200 healthy controls. The association between eNOS polymorphisms and preeclampsia was evaluated by performing genotyping for the eNOS variants and calculating odds ratios (OR) and 95% confidence intervals. The plasma nitrite concentration in participants was determined to examine how 3 eNOS polymorphisms affect plasma nitric oxide (NO) concentrations in pregnant women.
The frequencies of both the variant 298Asp allele and eNOS 4a allele were significantly lower in preeclamptic women than in the control group and had a significantly lower OR. The variant 298Asp allele and eNOS 4a are strongly associated with higher plasma NO concentrations in pregnant women.
Polymorphisms in the eNOS gene may be protective against preeclampsia in a Chinese population, and this protective effect may be associated with NO formation in plasma in pregnant women.
背景/目的：内皮型一氧化氮合酶（eNOS）基因已被先前的研究列为先兆子痫易感性的候选基因。本研究调查了内皮型一氧化氮合酶基因的 3 个多态性在中国大陆中国人群中的特定作用。
我们研究了内皮型一氧化氮合酶基因的 3 个常见多态性，即 4b/a、T-786C 和 Glu298Asp，在 220 例确诊为子痫前期的病例对照样本和 200 例健康对照中。通过对内皮型一氧化氮合酶变体进行基因分型，计算比值比（OR）和 95%置信区间，评估内皮型一氧化氮合酶多态性与子痫前期之间的关联。测定参与者的血浆亚硝酸盐浓度，以研究 3 种内皮型一氧化氮合酶多态性如何影响孕妇血浆中一氧化氮（NO）浓度。
子痫前期妇女的变体 298Asp 等位基因和内皮型一氧化氮合酶 4a 等位基因的频率明显低于对照组，OR 明显降低。变体 298Asp 等位基因和内皮型一氧化氮合酶 4a 与孕妇血浆中较高的 NO 浓度密切相关。
内皮型一氧化氮合酶基因的多态性可能对中国人群的子痫前期具有保护作用，这种保护作用可能与孕妇血浆中 NO 的形成有关。
