Ahmad Iqbal, Qadeer Kiranr, Hafeez Ambreen, Bano Rahila, Vaid Faiyaz H M
Department of Pharmaceutical Chemistry, Baqai Institute of Pharmaceutical Sciences, Baqai Medical University, Toll Plaza, Super Highway, Gadap Road, Karachi, Pakistan.
Department of Biochemistry, Biophysics Research Unit, University of Karachi, Karachi Pakistan.
Pak J Pharm Sci. 2014 Mar;27(2):209-15.
The simultaneous determination of cyanocobalamin (CC), hydroxocobalamin (HC) and ascorbic acid (AA) in aqueous solution has been achieved by a multicomponent spectrometric method. CC undergoes photolysis in acidic and alkaline media to form HC and the reaction is enhanced in the presence of AA. The method has been used to evaluate the kinetics of photodegradation reactions of the vitamin. CC, HC and AA present in the photolyzed solutions have been determined by absorbance measurement at 550, 525 and 265 nm at pH 4.0. These wavelengths correspond to the absorption maxima of the three substances and thus provide high specificity and sensitivity to the method. The method has been validated with respect to various parameters relating to the analytical performance characteristics. The recovery of the method for the three compounds ranges from 97.1-103.0% with a RSD value of ±3%. The accuracy of the method is shown by the linearity of the kinetic plots in the concentration range studied. The method is simple, rapid and convenient for the proposed work.
采用多组分光谱法实现了水溶液中钴胺素（CC）、羟钴胺素（HC）和抗坏血酸（AA）的同时测定。CC在酸性和碱性介质中发生光解形成HC，且在AA存在下反应会增强。该方法已用于评估维生素光降解反应的动力学。通过在pH 4.0时于550、525和265 nm处测量吸光度，测定了光解溶液中存在的CC、HC和AA。这些波长对应于三种物质的吸收最大值，因此为该方法提供了高特异性和灵敏度。该方法已针对与分析性能特征相关的各种参数进行了验证。该方法对三种化合物的回收率在97.1 - 103.0%之间，相对标准偏差（RSD）值为±3%。在所研究的浓度范围内，动力学曲线的线性表明了该方法的准确性。该方法对于所提出的工作而言简单、快速且方便。
