Hermansen K
Second University Clinic of Internal Medicine, Aarhus Kommunehospital, Denmark.
Acta Endocrinol (Copenh). 1988 Sep;119(1):91-8. doi: 10.1530/acta.0.1190091.
Galanin is a 29 amino acid peptide which has been found in intrapancreatic nerves. The effects of galanin, adrenergic and cholinergic blockade as well as somatostatin on the hormone release from the isolated perfused dog pancreas were studied. It was found that galanin dose-dependently inhibited insulin (P less than 0.001) and somatostatin (P less than 0.001) but not glucagon secretion at normal glucose levels. The lowest galanin concentration that caused a significant suppression of insulin and somatostatin secretion was 10(-11) and 10(-10) mol/l, respectively. Similar effects were evident during stimulation with 2.5 mmol/l arginine. Galanin (10(-9) mol/l) caused a more pronounced inhibition of insulin and somatostatin secretion at high (10 mmol/l) and normal (5 mmol/l) than at low glucose (1.3 mmol/l). In contrast, suppression of the glucagon secretion was only seen at low glucose (1.3 mmol/l). Perfusion of 10(-6) mol/l of atropine, phentolamine and propranolol had no effect on the galanin-mediated (10(-10) mol/l) inhibition of insulin and somatostatin secretion. Galanin (10(-12)-10(-10) mol/l) and somatostatin (10(-12)-10(-10) mol/l) were equipotent in inhibiting insulin secretion whereas only somatostatin exerted a suppression of the glucagon secretion at normal glucose. Thus, galanin exerts a differential effect on islet hormone secretion and may participate in the hormonal control of insulin, glucagon and somatostatin secretion.
甘丙肽是一种由29个氨基酸组成的肽，已在胰腺内神经中发现。研究了甘丙肽、肾上腺素能和胆碱能阻断以及生长抑素对离体灌注犬胰腺激素释放的影响。结果发现，在正常血糖水平下，甘丙肽剂量依赖性地抑制胰岛素（P<0.001）和生长抑素（P<0.001）分泌，但不影响胰高血糖素分泌。导致胰岛素和生长抑素分泌显著抑制的最低甘丙肽浓度分别为10^(-11)和10^(-10)mol/L。在2.5mmol/L精氨酸刺激期间也有类似的作用。甘丙肽（10^(-9)mol/L）在高血糖（10mmol/L）和正常血糖（5mmol/L）时比低血糖（1.3mmol/L）时更显著地抑制胰岛素和生长抑素分泌。相反，仅在低血糖（1.3mmol/L）时观察到胰高血糖素分泌受抑制。灌注10^(-6)mol/L的阿托品、酚妥拉明和普萘洛尔对甘丙肽介导的（10^(-10)mol/L）胰岛素和生长抑素分泌抑制无影响。甘丙肽（10^(-12)-10^(-10)mol/L）和生长抑素（10^(-12)-10^(-10)mol/L）在抑制胰岛素分泌方面具有同等效力，而在正常血糖时只有生长抑素能抑制胰高血糖素分泌。因此，甘丙肽对胰岛激素分泌具有差异作用，可能参与胰岛素、胰高血糖素和生长抑素分泌的激素调控。
