Lindskog S, Ahrén B, Dunning B E, Sundler F
Department of Pharmacology, Sandoz Research Institute, East Hanover, NJ 07936.
Cell Tissue Res. 1991 May;264(2):363-8. doi: 10.1007/BF00313975.
Galanin-containing nerve fibers have previously been observed in the human, dog, and pig pancreas. Whether the mouse and rat pancreas also contain galanin nerve fibers has been a matter of debate. Therefore, we examined the distribution of galanin in the mouse and the rat pancreas. Further, the possible localization of galanin to adrenergic nerves was studied using sequential immunostaining for galanin and tyrosine hydroxylase (TH). In the mouse pancreas, numerous galanin-immunoreactive (GIR) nerve fibers occurred around blood vessels. They were less numerous in the exocrine parenchyma and in association with the islets. In contrast, in the rat pancreas, only a few GIR nerves were found. They were located around blood vessels and scattered in the exocrine parenchyma. Occasionally, GIR nerves were also observed in the islets. There was a dense distribution of TH-immunoreactive fibers in both the mouse and the rat pancreas. Sequential immunostaining revealed co-localization of galanin and TH immunoreactivity in nerve fibers in both the mouse and the rat pancreas. Following chemical sympathectomy using 6-hydroxydopamine (6-OHDA), not all GIR nerves disappeared. In the mouse pancreas a remaining population of galanin nerves was found around blood vessels, and occasionally in the islets. In the rat pancreas, a few GIR nerves were seen also after chemical sympathectomy. We conclude that intrapancreatic GIR nerves also occur in the mouse and the rat. These findings suggest that many of the GIR nerves are adrenergic but that non-adrenergic, possibly intrinsic or sensory GIR nerves exist as well in both the mouse and the rat pancreas.
此前已在人类、狗和猪的胰腺中观察到含甘丙肽的神经纤维。小鼠和大鼠的胰腺是否也含有甘丙肽神经纤维一直存在争议。因此，我们研究了甘丙肽在小鼠和大鼠胰腺中的分布。此外，利用甘丙肽和酪氨酸羟化酶（TH）的连续免疫染色研究了甘丙肽在肾上腺素能神经上的可能定位。在小鼠胰腺中，许多甘丙肽免疫反应性（GIR）神经纤维出现在血管周围。它们在外分泌实质和与胰岛相关的部位数量较少。相比之下，在大鼠胰腺中，仅发现少数GIR神经。它们位于血管周围并散在于外分泌实质中。偶尔，在胰岛中也观察到GIR神经。在小鼠和大鼠胰腺中均有密集分布的TH免疫反应性纤维。连续免疫染色显示，在小鼠和大鼠胰腺的神经纤维中，甘丙肽和TH免疫反应性共定位。使用6-羟基多巴胺（6-OHDA）进行化学交感神经切除术后，并非所有GIR神经都消失。在小鼠胰腺中，在血管周围发现了剩余的甘丙肽神经群体，偶尔在胰岛中也有发现。在大鼠胰腺中，化学交感神经切除术后也可见少数GIR神经。我们得出结论，胰腺内GIR神经在小鼠和大鼠中也存在。这些发现表明，许多GIR神经是肾上腺素能的，但在小鼠和大鼠胰腺中也存在非肾上腺素能的、可能是内在的或感觉性的GIR神经。
