Yang Qing-tao, Gu Jiang, Zhang Yong-chun, Zhu Zhi-hui, Yang Yong-an, Wang Nan, Zhu Qing-liang
Department of Urologic Surgery, Affiliated Hospital, Guiyang Medical College, Guiyang 550004, China.
Department of Urologic Surgery, Jiangdu People's Hospital, Yangzhou, Jiangsu 225200, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2014 Feb;36(1):12-9. doi: 10.3881/j.issn.1000-503X.2014.01.003.
To explore the effects of stanniocalcin-1 (STC-1) and hypoxia-inducible factor-1α (HIF-1α) on the calcium and thus on the mitochondrial membrane potential (Δψm) in renal carcinoma cells.
We successfully established the renal carcinoma cell models with high HIF-1α gene expression. After various concentrations of STC-1 solutions were added to the culture medium, the proliferation of cells, expressions of HIF-1α and STC-1, levels of Ca(2+), Δψm, and mPTP were detected by MTT, RT-PCR, ELISA, fluorescence spectrophotometry, and ultraviolet spectrophotometry, respectively.
The proliferation of renal carcinoma cells and Δψm were improved after HIF-1α gene transfection, STC-1 protein intervention, and STC-1 protein intervention after gene transfection. While the intracellular Ca(2+) level and mPTP were decreased significantly (P<0.05), all the changes were intensified with the gradual increase of STC-1. However, the increasing trend of cell proliferation gradually declined.
HIF-1α may participate in malignant proliferation of renal carcinoma cells by promoting STC-1 proliferation or down-regulating Ca(2+); however, such an effect may be gradually attenuated due to the inhibitory effect of STC-1 on HIF-1α.
探讨鲽鱼钙蛋白-1（STC-1）和缺氧诱导因子-1α（HIF-1α）对肾癌细胞钙含量及线粒体膜电位（Δψm）的影响。
成功建立HIF-1α基因高表达的肾癌细胞模型。向培养基中加入不同浓度的STC-1溶液后，分别采用MTT法、RT-PCR法、ELISA法、荧光分光光度法及紫外分光光度法检测细胞增殖、HIF-1α和STC-1的表达、Ca(2+)水平、Δψm及线粒体通透性转换孔（mPTP）。
HIF-1α基因转染、STC-1蛋白干预及基因转染后STC-1蛋白干预均可使肾癌细胞增殖及Δψm升高，细胞内Ca(2+)水平及mPTP显著降低（P<0.05），且随着STC-1浓度的逐渐升高，上述变化均增强，但细胞增殖的上升趋势逐渐减弱。
HIF-1α可能通过促进STC-1增殖或下调Ca(2+)参与肾癌细胞的恶性增殖；然而，由于STC-1对HIF-1α的抑制作用，这种效应可能会逐渐减弱。
