Pauli G, Hoyet C, Tenabene A, le Mao J, Thierry R, Bessot J C
Service de Pneumologie, Centre Hospitalier Universitaire, Strasbourg, France.
Clin Allergy. 1988 Jul;18(4):383-92. doi: 10.1111/j.1365-2222.1988.tb02886.x.
Guanine is the major nitrogenous waste product in arachnids. It may serve as an indicator for allergenic mite faecal pellets. The present study assesses the correlation between the mit allergenicity of different house dust (HD) samples and their guanine content. Guanine content in HD samples was evaluated either by the Acarex-Test or determined quantitatively, especially for in-vitro methods. Using intradermal tests in selectively sensitized Dermatophagoides pteronyssinus (Dpt) patients, we evaluated the mite allergenicity of eight extracts obtained from HD samples with varying guanine contents. The weal area appeared to be proportional to the HD samples' guanine content. A three-fold increase in skin reactivity was observed when the guanine content varied from 0.06% to more than 1%. Patients allergic to HD but not to Dpt had no significant reactions with HD extract prepared from a guanine-rich HD sample. For eighteen different HD samples coupled to paper discs, the percentage Dpt RAST binding values obtained with a Dpt-selectively sensitized patient serum pool was correlated with the guanine concentrations of HD samples (P less than 0.01). The potencies of different HD extracts obtained from HD samples that varied in guanine content were also clearly distinguished on histamine-release titration curves. House dust obtained from homes at high altitude or from hospitals was generally guanine free. The HD sample guanine contents in homes where twelve Dpt-sensitized patients experienced symptoms were significantly higher than in homes where these patients were symptom free. The guanine colour test, a quick and easy technique, represents an important new development in indoor environmental investigations. It makes it possible to demonstrate mite faecal exposure and can be used to monitor the effectiveness of mite allergen avoidance measures.
鸟嘌呤是蛛形纲动物主要的含氮废物产物。它可用作致敏螨粪便颗粒的一个指标。本研究评估了不同室内灰尘（HD）样本的螨致敏性与其鸟嘌呤含量之间的相关性。HD样本中的鸟嘌呤含量通过阿卡里克斯试验进行评估，或进行定量测定，特别是针对体外方法。通过对选择性致敏的粉尘螨（Dpt）患者进行皮内试验，我们评估了从鸟嘌呤含量不同的HD样本中获得的八种提取物的螨致敏性。风团面积似乎与HD样本的鸟嘌呤含量成正比。当鸟嘌呤含量从0.06%变化到超过1%时，观察到皮肤反应性增加了三倍。对HD过敏但对Dpt不过敏的患者，用富含鸟嘌呤的HD样本制备的HD提取物没有明显反应。对于与纸盘相连的18个不同HD样本，用Dpt选择性致敏患者血清池获得的Dpt RAST结合值百分比与HD样本的鸟嘌呤浓度相关（P小于0.01）。从鸟嘌呤含量不同的HD样本中获得的不同HD提取物的效价在组胺释放滴定曲线上也有明显区分。从高海拔地区家庭或医院获得的室内灰尘通常不含鸟嘌呤。在12名对Dpt致敏的患者出现症状的家庭中，HD样本的鸟嘌呤含量显著高于这些患者无症状的家庭。鸟嘌呤颜色测试是一种快速简便的技术，是室内环境调查中的一项重要新进展。它能够证明螨粪便暴露情况，并可用于监测螨过敏原回避措施的有效性。
