Mackie E J, Tucker R P, Halfter W, Chiquet-Ehrismann R, Epperlein H H
Friedrich Miescher-Institut, Basel, Switzerland.
Development. 1988 Jan;102(1):237-50. doi: 10.1242/dev.102.1.237.
The distribution of the extracellular matrix (ECM) glycoprotein, tenascin, has been compared with that of fibronectin in neural crest migration pathways of Xenopus laevis, quail and rat embryos. In all species studied, the distribution of tenascin, examined by immunohistochemistry, was more closely correlated with pathways of migration than that of fibronectin, which is known to be important for neural crest migration. In Xenopus laevis embryos, anti-tenascin stained the dorsal fin matrix and ECM along the ventral route of migration, but not the ECM found laterally between the ectoderma and somites where neural crest cells do not migrate. In quail embryos, the appearance of tenascin in neural crest pathways was well correlated with the anterior-to-posterior wave of migration. The distribution of tenascin within somites was compared with that of the neural crest marker, HNK-1, in quail embryos. In the dorsal halves of quail somites which contained migrating neural crest cells, the predominant tenascin staining was in the anterior halves of the somites, codistributed with the migrating cells. In rat embryos, tenascin was detectable in the somites only in the anterior halves. Tenascin was not detectable in the matrix of cultured quail neural crest cells, but was in the matrix surrounding somite and notochord cells in vitro. Neural crest cells cultured on a substratum of tenascin did not spread and were rounded. We propose that tenascin is an important factor controlling neural crest morphogenesis, perhaps by modifying the interaction of neural crest cells with fibronectin.
已将细胞外基质（ECM）糖蛋白腱生蛋白的分布与纤连蛋白在非洲爪蟾、鹌鹑和大鼠胚胎神经嵴迁移途径中的分布进行了比较。在所研究的所有物种中，通过免疫组织化学检测的腱生蛋白分布与迁移途径的相关性比纤连蛋白更紧密，而纤连蛋白已知对神经嵴迁移很重要。在非洲爪蟾胚胎中，抗腱生蛋白抗体染色了背鳍基质和沿腹侧迁移途径的细胞外基质，但未染色神经嵴细胞不迁移的外胚层和体节之间的外侧细胞外基质。在鹌鹑胚胎中，腱生蛋白在神经嵴途径中的出现与从前到后的迁移波密切相关。在鹌鹑胚胎中，将体节内腱生蛋白的分布与神经嵴标记物HNK-1的分布进行了比较。在含有迁移神经嵴细胞的鹌鹑体节的背半部分，主要的腱生蛋白染色位于体节的前半部分，与迁移细胞共分布。在大鼠胚胎中，仅在前半部分的体节中可检测到腱生蛋白。在培养的鹌鹑神经嵴细胞的基质中未检测到腱生蛋白，但在体外体节和脊索细胞周围的基质中可检测到。在腱生蛋白基质上培养的神经嵴细胞不铺展且呈圆形。我们提出腱生蛋白是控制神经嵴形态发生的重要因素，可能是通过改变神经嵴细胞与纤连蛋白的相互作用来实现的。
