Anstrom John A, Mackie Eleanor J, Tucker Richard P
Department of Neurobiology and Anatomy, The Bowman Gray School of Medicine, Wake Forest University, 300 S. Hawthorne Road, 27103, Winston-Salem, NC, USA.
Departement Forschung, Kantonspital Basel, 4031, Basel, Switzerland.
Rouxs Arch Dev Biol. 1990 Nov;199(3):169-173. doi: 10.1007/BF01681490.
We have used polyclonal antisera raised against vertebrate tenascin to identify and localize tenascin-like proteins in the developing sea urchin. These antisera recognize high-molecular weight proteins on immunoblots of sea urchin embryo homogenates that are similar in size and appearance to tenascin from vertebrates. These proteins appear as a doublet with an apparent molecular weight of 150 kDa and a larger, broad band with an apparent molecular weight of 350 kDa. Whole mounts of sea urchin embryos and larvae were stained with one of these antisera. The anti-tenascin stained the surface of primary mesenchyme cells during their phase of active migration. This staining was sensitive to detergent, suggesting that the protein recognized by the antiserum was associated with the cell surface. During later stages of development, the bulk of the antitenascin staining was found dispersed throughout the blastocoel matrix, and was no longer sensitive to detergent. We conclude that sea urchins express tenascin-like proteins during early stages of development, and that these proteins may play a role associated with primary mesenchyme cell morphogenesis.
我们使用针对脊椎动物腱生蛋白产生的多克隆抗血清，来鉴定和定位发育中的海胆体内的腱生蛋白样蛋白。这些抗血清在海胆胚胎匀浆的免疫印迹上识别出高分子量蛋白，其大小和外观与脊椎动物的腱生蛋白相似。这些蛋白表现为一条表观分子量为150 kDa的双峰带和一条表观分子量为350 kDa的更大的宽带。用其中一种抗血清对海胆胚胎和幼虫的整体标本进行染色。抗腱生蛋白在初级间充质细胞活跃迁移阶段对其表面进行了染色。这种染色对去污剂敏感，表明抗血清识别的蛋白与细胞表面相关。在发育后期，大部分抗腱生蛋白染色发现分散在整个囊胚腔基质中，并且不再对去污剂敏感。我们得出结论，海胆在发育早期表达腱生蛋白样蛋白，并且这些蛋白可能在初级间充质细胞形态发生中发挥相关作用。
