College of Biomedical Engineering & Instrument Science, Zhejiang University, 38 Zheda Road, Hangzhou, Zhejiang, China.
College of Biomedical Engineering & Instrument Science, Zhejiang University, 38 Zheda Road, Hangzhou, Zhejiang, China.
Gene. 2014 May 1;540(2):201-9. doi: 10.1016/j.gene.2014.02.037. Epub 2014 Feb 26.
Taxus chinensis var. mairei (Taxaceae) is a domestic variety of yew species in local China. This plant is one of the sources for paclitaxel, which is a promising antineoplastic chemotherapy drugs during the last decade. We have sequenced the complete nucleotide sequence of the chloroplast (cp) genome of T. chinensis var. mairei. The T. chinensis var. mairei cp genome is 129,513 bp in length, with 113 single copy genes and two duplicated genes (trnI-CAU, trnQ-UUG). Among the 113 single copy genes, 9 are intron-containing. Compared to other land plant cp genomes, the T. chinensis var. mairei cp genome has lost one of the large inverted repeats (IRs) found in angiosperms, fern, liverwort, and gymnosperm such as Cycas revoluta and Ginkgo biloba L. Compared to related species, the gene order of T. chinensis var. mairei has a large inversion of ~110kb including 91 genes (from rps18 to accD) with gene contents unarranged. Repeat analysis identified 48 direct and 2 inverted repeats 30 bp long or longer with a sequence identity greater than 90%. Repeated short segments were found in genes rps18, rps19 and clpP. Analysis also revealed 22 simple sequence repeat (SSR) loci and almost all are composed of A or T.
中国红豆杉（红豆杉科）是中国本土的一种紫杉物种。这种植物是紫杉醇的来源之一，紫杉醇是过去十年中很有前途的一种抗肿瘤化疗药物。我们已经对中国红豆杉 var. mairei 的叶绿体（cp）基因组的完整核苷酸序列进行了测序。中国红豆杉 var. mairei 的 cp 基因组长 129513bp，包含 113 个单拷贝基因和两个重复基因（trnI-CAU、trnQ-UUG）。在 113 个单拷贝基因中，有 9 个是内含子基因。与其他陆地植物 cp 基因组相比，中国红豆杉 var. mairei 的 cp 基因组丢失了被子植物、蕨类植物、苔藓植物和裸子植物（如苏铁和银杏）中发现的一个较大的反向重复（IR）。与相关物种相比，中国红豆杉 var. mairei 的基因排列发生了较大的倒位，大约 110kb 的区域包含 91 个基因（从 rps18 到 accD），基因内容没有排列。重复分析确定了 48 个直接重复和 2 个 30bp 长或更长的反向重复，其序列同一性大于 90%。在 rps18、rps19 和 clpP 基因中发现了重复的短片段。分析还揭示了 22 个简单序列重复（SSR）位点，几乎所有都是由 A 或 T 组成的。
