从红豆杉（Taxaceae）叶绿体全基因组开发的叶绿体微卫星标记。
Chloroplast microsatellite markers for developed from the whole chloroplast genome of var. (Taxaceae).
作者信息
Deng Qi, Zhang Hanrui, He Yipeng, Wang Ting, Su Yingjuan
机构信息
School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, People's Republic of China; School of Medicine, Guangxi University of Science and Technology, Liuzhou 545005, People's Republic of China.
School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, People's Republic of China.
出版信息
Appl Plant Sci. 2017 Mar 14;5(3). doi: 10.3732/apps.1600153. eCollection 2017 Mar.
Abstract
PREMISE OF THE STUDY
(Taxaceae) is an old rare species endemic to China that has adapted well to ecological heterogeneity with high genetic diversity in its nuclear genome. However, the genetic variation in its chloroplast genome is unknown.
METHODS AND RESULTS
Eighteen chloroplast microsatellite markers (cpSSRs) were developed from the whole chloroplast genome of var. and successfully amplified in four populations and one var. population. Of these loci, 10 were polymorphic in , whereas six were polymorphic in var. . The unbiased haploid diversity per locus ranged from 0.000 to 0.641 and 0.000 to 0.545 for and var. , respectively.
CONCLUSIONS
The 18 cpSSRs will be used to further investigate the chloroplast genetic structure and adaptive evolution in populations.
摘要
研究前提
（红豆杉科）是中国特有的古老珍稀物种，已很好地适应了生态异质性，其核基因组具有高遗传多样性。然而，其叶绿体基因组中的遗传变异尚不清楚。
方法与结果
从 变种的整个叶绿体基因组中开发了 18 个叶绿体微卫星标记（cpSSRs），并在 4 个 种群和 1 个 变种种群中成功扩增。在这些位点中，10 个在 中具有多态性，而 6 个在 变种中具有多态性。每个位点的无偏单倍体多样性在 中分别为 0.000 至 0.641，在 变种中为 0.000 至 0.545。
结论
这 18 个 cpSSRs 将用于进一步研究 种群的叶绿体遗传结构和适应性进化。
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