• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Microplate-based screening for small molecule inhibitors of neuropilin-2/vascular endothelial growth factor-C interactions.基于微孔板筛选神经纤毛蛋白-2/血管内皮生长因子-C相互作用的小分子抑制剂。
Anal Biochem. 2014 May 15;453:4-6. doi: 10.1016/j.ab.2014.02.017. Epub 2014 Feb 26.
2
Structural basis for VEGF-C binding to neuropilin-2 and sequestration by a soluble splice form.血管内皮生长因子C（VEGF-C）与神经纤毛蛋白-2（neuropilin-2）结合及被可溶性剪接形式隔离的结构基础。
Structure. 2015 Apr 7;23(4):677-87. doi: 10.1016/j.str.2015.01.018. Epub 2015 Mar 5.
3
Design, synthesis, and evaluation of a novel benzamidine-based inhibitor of VEGF-C binding to Neuropilin-2.设计、合成及评价一种新型苯甲脒类抑制剂，其可与 Neuropilin-2 结合抑制 VEGF-C。
Bioorg Chem. 2020 Jul;100:103856. doi: 10.1016/j.bioorg.2020.103856. Epub 2020 Apr 16.
4
Prognostic significance of VEGF-C, semaphorin 3F, and neuropilin-2 expression in oral squamous cell carcinomas and their relationship with lymphangiogenesis.血管内皮生长因子-C、信号素3F和神经纤毛蛋白-2在口腔鳞状细胞癌中的表达及其与淋巴管生成的关系的预后意义
J Surg Oncol. 2015 Mar 15;111(4):382-8. doi: 10.1002/jso.23842. Epub 2014 Dec 4.
5
Neuropilin-2 interacts with VEGFR-2 and VEGFR-3 and promotes human endothelial cell survival and migration.神经纤毛蛋白-2与血管内皮生长因子受体-2和血管内皮生长因子受体-3相互作用，促进人内皮细胞的存活和迁移。
Blood. 2006 Aug 15;108(4):1243-50. doi: 10.1182/blood-2005-11-4447. Epub 2006 Apr 18.
6
Neuropilin-2 mediates VEGF-C-induced lymphatic sprouting together with VEGFR3.神经纤毛蛋白-2 与 VEGFR3 共同介导 VEGF-C 诱导的淋巴管生成。
J Cell Biol. 2010 Jan 11;188(1):115-30. doi: 10.1083/jcb.200903137.
7
Neuropilin-2 mediates lymphangiogenesis of colorectal carcinoma via a VEGFC/VEGFR3 independent signaling.神经纤毛蛋白-2通过VEGFC/VEGFR3非依赖信号通路介导结直肠癌的淋巴管生成。
Cancer Lett. 2015 Mar 28;358(2):200-209. doi: 10.1016/j.canlet.2014.12.046. Epub 2014 Dec 24.
8
Pathway-related molecules of VEGFC/D-VEGFR3/NRP2 axis in tumor lymphangiogenesis and lymphatic metastasis.肿瘤淋巴管生成和淋巴转移中VEGFC/D-VEGFR3/NRP2轴的通路相关分子
Clin Chim Acta. 2016 Oct 1;461:165-71. doi: 10.1016/j.cca.2016.08.008. Epub 2016 Aug 12.
9
Concurrent Expression of VEGF-C and Neuropilin-2 Is Correlated with Poor Prognosis in Glioblastoma.血管内皮生长因子 C 与神经纤毛蛋白-2 的共表达与胶质母细胞瘤的不良预后相关。
Tohoku J Exp Med. 2016 Feb;238(2):85-91. doi: 10.1620/tjem.238.85.
10
Molecular controls of lymphatic VEGFR3 signaling.淋巴管内皮 VEGFR3 信号的分子调控
Arterioscler Thromb Vasc Biol. 2015 Feb;35(2):421-9. doi: 10.1161/ATVBAHA.114.304881. Epub 2014 Dec 18.

引用本文的文献

1
Role of Neuropilin-2-mediated signaling axis in cancer progression and therapy resistance.神经纤毛蛋白-2 介导的信号轴在癌症进展和治疗耐药中的作用。
Cancer Metastasis Rev. 2022 Sep;41(3):771-787. doi: 10.1007/s10555-022-10048-0. Epub 2022 Jul 1.
2
MiR-377-3p inhibits atherosclerosis-associated vascular smooth muscle cell proliferation and migration via targeting neuropilin2.miR-377-3p 通过靶向神经纤毛蛋白 2 抑制动脉粥样硬化相关血管平滑肌细胞增殖和迁移。
Biosci Rep. 2020 Jun 26;40(6). doi: 10.1042/BSR20193425.
3
Design, synthesis, and evaluation of a novel benzamidine-based inhibitor of VEGF-C binding to Neuropilin-2.设计、合成及评价一种新型苯甲脒类抑制剂，其可与 Neuropilin-2 结合抑制 VEGF-C。
Bioorg Chem. 2020 Jul;100:103856. doi: 10.1016/j.bioorg.2020.103856. Epub 2020 Apr 16.
4
Deletion of neuropilin 2 enhances detrusor contractility following bladder outlet obstruction.神经纤毛蛋白 2 的缺失增强了膀胱出口梗阻后逼尿肌的收缩性。
JCI Insight. 2017 Feb 9;2(3):e90617. doi: 10.1172/jci.insight.90617.
5
The non-canonical role of vascular endothelial growth factor-C axis in cancer progression.血管内皮生长因子-C轴在癌症进展中的非经典作用。
Exp Biol Med (Maywood). 2015 Jun;240(6):718-24. doi: 10.1177/1535370215583802. Epub 2015 Apr 16.
6
Structural basis for VEGF-C binding to neuropilin-2 and sequestration by a soluble splice form.血管内皮生长因子C（VEGF-C）与神经纤毛蛋白-2（neuropilin-2）结合及被可溶性剪接形式隔离的结构基础。
Structure. 2015 Apr 7;23(4):677-87. doi: 10.1016/j.str.2015.01.018. Epub 2015 Mar 5.

本文引用的文献

1
Mechanistic basis for the potent anti-angiogenic activity of semaphorin 3F.神经信号蛋白 3F 发挥强大抗血管生成活性的机制基础。
Biochemistry. 2013 Oct 29;52(43):7551-8. doi: 10.1021/bi401034q. Epub 2013 Oct 18.
2
Transient mammalian cell transfection with polyethylenimine (PEI).用聚乙烯亚胺（PEI）进行瞬时哺乳动物细胞转染。
Methods Enzymol. 2013;529:227-40. doi: 10.1016/B978-0-12-418687-3.00018-5.
3
Effect of C-terminal sequence on competitive semaphorin binding to neuropilin-1.C 末端序列对神经纤毛蛋白-1与竞争 semaphorin 结合的影响。
J Mol Biol. 2013 Nov 15;425(22):4405-14. doi: 10.1016/j.jmb.2013.07.017. Epub 2013 Jul 17.
4
Mechanism of selective VEGF-A binding by neuropilin-1 reveals a basis for specific ligand inhibition.神经纤毛蛋白-1 选择性结合 VEGF-A 的机制揭示了特定配体抑制的基础。
PLoS One. 2012;7(11):e49177. doi: 10.1371/journal.pone.0049177. Epub 2012 Nov 8.
5
Function of members of the neuropilin family as essential pleiotropic cell surface receptors.神经钙黏蛋白家族成员作为必需的多功能细胞表面受体的功能。
Biochemistry. 2012 Nov 27;51(47):9437-46. doi: 10.1021/bi3012143. Epub 2012 Nov 14.
6
Studies of vascular endothelial growth factor in asthma and chronic obstructive pulmonary disease.哮喘和慢性阻塞性肺疾病中血管内皮生长因子的研究。
Proc Am Thorac Soc. 2011 Nov;8(6):512-5. doi: 10.1513/pats.201102-018MW.
7
Furin processing of semaphorin 3F determines its anti-angiogenic activity by regulating direct binding and competition for neuropilin.弗林蛋白酶对半信号素 3F 的加工通过调节直接结合和与神经纤毛蛋白的竞争来决定其抗血管生成活性。
Biochemistry. 2010 May 18;49(19):4068-75. doi: 10.1021/bi100327r.
8
Small molecule inhibitors of the neuropilin-1 vascular endothelial growth factor A (VEGF-A) interaction.神经纤毛蛋白 1-血管内皮生长因子 A（VEGF-A）相互作用的小分子抑制剂。
J Med Chem. 2010 Mar 11;53(5):2215-26. doi: 10.1021/jm901755g.
9
Structure-function analysis of the antiangiogenic ATWLPPR peptide inhibiting VEGF(165) binding to neuropilin-1 and molecular dynamics simulations of the ATWLPPR/neuropilin-1 complex.抗血管生成ATWLPPR肽抑制VEGF(165)与神经纤毛蛋白-1结合的结构-功能分析及ATWLPPR/神经纤毛蛋白-1复合物的分子动力学模拟
Peptides. 2007 Dec;28(12):2397-402. doi: 10.1016/j.peptides.2007.09.013. Epub 2007 Sep 29.
10
Structural basis for ligand and heparin binding to neuropilin B domains.配体和肝素与神经纤毛蛋白B结构域结合的结构基础。
Proc Natl Acad Sci U S A. 2007 Apr 10;104(15):6152-7. doi: 10.1073/pnas.0700043104. Epub 2007 Apr 3.

基于微孔板筛选神经纤毛蛋白-2/血管内皮生长因子-C相互作用的小分子抑制剂。

Microplate-based screening for small molecule inhibitors of neuropilin-2/vascular endothelial growth factor-C interactions.

作者信息

Parker Matthew W, Vander Kooi Craig W

机构信息

Center for Structural Biology, Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40536, USA.

Center for Structural Biology, Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40536, USA.

出版信息

Anal Biochem. 2014 May 15;453:4-6. doi: 10.1016/j.ab.2014.02.017. Epub 2014 Feb 26.

DOI:10.1016/j.ab.2014.02.017
PMID:24583243
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4036672/
Abstract

Vascular endothelial growth factor-C (VEGF-C) is a secreted growth factor essential for lymphangiogenesis. VEGF-C functions in both physiological and pathological lymphangiogenesis, particularly in tumor metastasis, making it an attractive therapeutic target. Members of two families of cell surface receptors transduce VEGF-C signals: neuropilin-2 (Nrp2) and VEGF-receptor (VEGFR)-2/3. Nrp2 is a promising target for inhibition because it is highly expressed in lymphatic vessels. Here we describe a microplate-based assay for discovery of VEGF-C/Nrp2 inhibitors. We optimize this assay for use in screening an inhibitor library and identify three novel Nrp2/VEGF-C binding inhibitors from the National Institutes of Health (NIH) Clinical Collection small molecule library.

摘要

血管内皮生长因子-C（VEGF-C）是一种对淋巴管生成至关重要的分泌型生长因子。VEGF-C在生理性和病理性淋巴管生成中均发挥作用，尤其是在肿瘤转移方面，这使其成为一个有吸引力的治疗靶点。两类细胞表面受体家族的成员可转导VEGF-C信号：神经纤毛蛋白-2（Nrp2）和血管内皮生长因子受体（VEGFR）-2/3。Nrp2是一个很有前景的抑制靶点，因为它在淋巴管中高度表达。在此，我们描述了一种基于微孔板的检测方法，用于发现VEGF-C/Nrp2抑制剂。我们对该检测方法进行了优化，以用于筛选抑制剂文库，并从美国国立卫生研究院（NIH）临床化合物库小分子文库中鉴定出三种新型的Nrp2/VEGF-C结合抑制剂。