鉴定控制水稻抽穗期的 QTLs LH8 和 EH3 之间上位性互作。
Characterization of epistatic interaction of QTLs LH8 and EH3 controlling heading date in rice.
机构信息
1] State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangdong Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou 510642, China [2] Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
1] State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangdong Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou 510642, China [2] College of Agronomy, Northwest Agriculture & Forestry University, Yangling, 712100, China.
出版信息
Sci Rep. 2014 Mar 3;4:4263. doi: 10.1038/srep04263.
Heading date is a critical trait for adaptation of rice to different cultivation areas and cropping seasons. We evaluated the heading dates of 1,123 chromosome segments substitution lines (CSSLs) in the genetic background of an elite rice variety Huajingxian74 (HJX74). A CSSL with the substituted segments from Zihui100 exhibited late heading under both natural long-day (NLD) and natural short-day (NSD) conditions, and the late heading phenotype was controlled by two novel epistatic loci on chromosome 8 and chromosome 3, respectively, termed LH8 and EH3. The function of EH3 was dependent on the LH8 genotype through epistatic interaction between EH3(Zihui100) and LH8(Zihui100) alleles. Genetic and molecular characterization revealed LH8 encodes a CCAAT-box-binding transcription factor with Heading date1 (Hd1)-binding activity and may delay flowering by repressing the expression of Early heading date1 (Ehd1). Our work provides a solid foundation for further study on gene interaction in heading date and has application in breeding rice with greater adaptability.
抽穗期是水稻适应不同种植区和种植季节的关键特性。我们评估了在优质水稻品种华粳 74（HJX74）遗传背景下的 1123 个染色体片段代换系（CSSLs）的抽穗期。来自紫辉 100 的一个代换片段的 CSSL 在自然长日（NLD）和自然短日（NSD）条件下均表现出晚抽穗，晚抽穗表型分别由第 8 号和第 3 号染色体上的两个新的上位性基因座控制，分别称为 LH8 和 EH3。EH3 的功能取决于 LH8 基因型，通过 EH3（紫辉 100）和 LH8（紫辉 100）等位基因之间的上位性相互作用。遗传和分子特征表明，LH8 编码一个具有 Hd1 结合活性的 CCAAT 框结合转录因子，可能通过抑制 Early heading date1（Ehd1）的表达来延迟开花。我们的工作为进一步研究抽穗期基因互作提供了坚实的基础，并在培育适应性更强的水稻方面具有应用价值。
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