Guangdong Key Lab of Plant Molecular Breeding, College of Agronomy, South China Agricultural University, Guangzhou, 510642, P. R. China.
College of Mathematics and Informatics, College of Software Engineering, South China Agricultural University, Guangzhou, 510642, P. R. China.
Sci Rep. 2018 Feb 15;8(1):3059. doi: 10.1038/s41598-018-20690-w.
Heading date directly determines the planting districts and seasons, and thus plays an important role for producing and introducing of varieties. Limited to the materials and methodologies, analysis of epistasis still presents an obvious challenge. This thesis estimated effectively four types of epistatic components among dual QTLs on heading date based on eight single segment substitution lines (SSSLs) in rice. The results confirmed that they carried truly with heading date QTLs. Eleven pairs of QTLs were with 50.0% of significant epistatic effects, of which additive-additive, additive-dominance or dominance-additive, and dominance-dominance interaction components occupied 40.9%, 50.0% and 59.1%, respectively. One QTL always interacted with multiple QTLs in various components. Several characteristics of epistasis on heading date were found that 1) different epistatic components had almost consistent directions; 2) dominance-dominance epistasis was perhaps most important in the four epistatic components; 3) epistasis was mostly positive, delaying rice heading; and 4) all epistatic components were seasonal sensitive. Two flowering pathways were further confirmed via a network constructed among these QTLs. These results have further confirmed the prevalence of epistatic interactions, deepened the understanding of genetic and flowering mechanism, and excavated several advantageous genes on heading date in rice.
标题日期直接决定种植区和季节，因此对品种的生产和引进起着重要作用。由于材料和方法的限制，上位性分析仍然是一个明显的挑战。本论文基于水稻的 8 个单片段替换系（SSSL），有效估计了双 QTL 对抽穗期的 4 种上位性成分。结果证实，它们确实携带了抽穗期 QTL。11 对 QTL 有 50.0%的显著上位性效应，其中加性-加性、加性-显性或显性-加性、显性-显性相互作用成分分别占 40.9%、50.0%和 59.1%。一个 QTL 总是在不同的成分中与多个 QTL 相互作用。在抽穗期上位性中发现了几个特征：1）不同的上位性成分几乎有一致的方向；2）显性-显性上位性可能是四个上位性成分中最重要的；3）上位性大多是阳性的，延迟了水稻的抽穗；4）所有的上位性成分都是季节性敏感的。通过这些 QTL 之间构建的网络进一步证实了两个开花途径。这些结果进一步证实了上位性相互作用的普遍性，深化了对遗传和开花机制的理解，并挖掘了水稻抽穗期的几个有利基因。
