Hemmati Matin, Mashayekhi Farhad, Firouzi Fareheh, Ashori Masoumeh, Mashayekhi Hamidreza
Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran.
Neurol Sci. 2014 Aug;35(8):1243-7. doi: 10.1007/s10072-014-1690-z. Epub 2014 Mar 1.
Many studies describe the biological effects of electromagnetic fields (EMF) including brain damages, neuronal migration and neurogenesis within the central nervous system. Neuronal cell produced in the neuroepithelium migrates along radial glial fibers into the cortical plate. Reelin, which is produced by Cajal-Retzius cells directs neuronal migration. It was shown that Disabled 1 (Dab1) functions downstream of reelin signal transduction pathway that directs the correct cytoarchitecture of the developing cortex. In this study, the EMF effects on total protein concentration (TPC), reelin and Dab1 expression in the developing cortex was studied. 30 pregnant Balb/c mice were separated into three groups: control (n = 10), EMF (n = 10) and SHAM groups (n = 10). The 15-day pregnant mice were placed inside the solenoid for a daily EMF exposure of 5 h for 3 consecutive days (15-17). The SHAM group was also located in the same coil with no exposure. Mice were sacrificed 24 h after the final exposure session. TPC, reelin and Dab1 expression were studied by Bio-Rad protein assay and western blot. No significant change in the TPC was seen in the EMF-treated cerebral cortex samples compared with those from the SHAM and control groups. It was also shown that the reelin and Dab1 expression increases in the EMF-treated cerebral cortex extracts as compared to controls and SHAM group. It is concluded that EMF may play important role in the neural cell migration by increasing reelin and Dab1 expression in the developing cortex.
许多研究描述了电磁场（EMF）的生物学效应，包括中枢神经系统内的脑损伤、神经元迁移和神经发生。神经上皮中产生的神经元细胞沿着放射状胶质纤维迁移到皮质板。由Cajal-Retzius细胞产生的Reelin指导神经元迁移。研究表明，Disabled 1（Dab1）在Reelin信号转导途径的下游发挥作用，该途径指导发育中皮质的正确细胞结构。在本研究中，研究了电磁场对发育中皮质中总蛋白浓度（TPC）、Reelin和Dab1表达的影响。30只怀孕的Balb/c小鼠被分为三组：对照组（n = 10）、电磁场组（n = 10）和假手术组（n = 10）。怀孕15天的小鼠被放置在螺线管内，每天暴露于电磁场5小时，连续3天（第15 - 17天）。假手术组也位于同一线圈中，但不进行暴露。在最后一次暴露后24小时处死小鼠。通过Bio-Rad蛋白质测定法和蛋白质印迹法研究TPC、Reelin和Dab1的表达。与假手术组和对照组相比，电磁场处理的大脑皮质样本中TPC没有显著变化。研究还表明，与对照组和假手术组相比，电磁场处理的大脑皮质提取物中Reelin和Dab1的表达增加。得出的结论是，电磁场可能通过增加发育中皮质中Reelin和Dab1的表达在神经细胞迁移中发挥重要作用。
