Noda Y, Kumasa S, Higashiyama H, Mori M
Department of Oral Surgery, Asahi University, School of Dentistry, Gifu, Japan.
Pathol Res Pract. 1988 Jun;183(3):284-91. doi: 10.1016/S0344-0338(88)80123-7.
A total of 34 cases (eccrine poroma: 2, cylindroma: 2, eccrine spiradenoma: 4, syringocystadenoma papilliferum: 1, hydroadenoma papilliferum: 1, clear cell hydroadenoma: 7, mixed tumour: 16) of sweat gland tumours of the skin were described in terms of immunohistochemical distribution of keratins using polyclonal anti-keratin antiserum (TK, detecting 41-56 KDa keratins) and monoclonal antibodies (KL1, 55-57 KDa; PKK1, 40, 45, 52.5 KDa). Keratin expression in eccrine poroma, spiradenoma and syringocystadenoma was similar to that in the ductal segment of normal sweat glands. Cylindroma showed usually slight staining for kertins. Tumour cells of hydroadenomas showed not so prominent staining for any of the keratins; however, histologically, tumour cells indicated marked variation, and the degree of keratin proteins also was different among these histological variants. Mixed tumours of the skin were strongly decorated with anti-keratin antibodies along the luminal surface cells of typical structures, while no staining occurred in outer side cells. Luminal tumour cells may be differentiated from secretory coil cells, whereas outer side cells may have a myoepithelial origin, as outer layer cells found in pleomorphic adenoma of salivary glands.
采用多克隆抗角蛋白抗血清（TK，检测41 - 56 kDa角蛋白）和单克隆抗体（KL1，55 - 57 kDa；PKK1，40、45、52.5 kDa），根据角蛋白的免疫组织化学分布描述了34例皮肤汗腺肿瘤（小汗腺汗孔瘤：2例；圆柱瘤：2例；小汗腺螺旋腺瘤：4例；乳头状汗管囊腺瘤：1例；乳头状 hydroadenoma：1例；透明细胞 hydroadenoma：7例；混合瘤：16例）。小汗腺汗孔瘤、螺旋腺瘤和乳头状汗管囊腺瘤中的角蛋白表达与正常汗腺导管段相似。圆柱瘤对角蛋白通常呈轻度染色。hydroadenomas的肿瘤细胞对任何角蛋白的染色都不那么明显；然而，在组织学上，肿瘤细胞显示出明显的变异，并且这些组织学变异之间角蛋白蛋白的程度也不同。皮肤混合瘤在典型结构的管腔表面细胞上被抗角蛋白抗体强烈标记，而外侧细胞无染色。管腔肿瘤细胞可能与分泌盘曲细胞不同，而外侧细胞可能起源于肌上皮，如同在涎腺多形性腺瘤中发现的外层细胞。
