Bosman F T
Department of Pathology, University of Limburg, Maastricht, The Netherlands.
Acta Histochem Suppl. 1988;35:27-32.
In this review the application of monoclonal antibodies for immunocytochemical purposes is discussed. An important basic principle is that from a methodological point of view that there are no essential differences between monoclonal and polyclonal antibody based immunocytochemistry. Specificity of the primary antibody is the most important prerequisite for reliable immunocytochemistry. Enzyme immunoassay, immunoblotting, antigen spot testing and tissue testing are the recommended procedures for specificity analysis. Studies of the most frequently used linking systems have shown that they are all suitable for use in conjunction with monoclonal antibodies. This also applies to the most important labels. It is concluded that monoclonal antibody based immunocytochemistry is one of the most valuable tools available in cell biology and pathology.
本综述讨论了单克隆抗体在免疫细胞化学中的应用。一个重要的基本原则是，从方法学角度来看，基于单克隆抗体和多克隆抗体的免疫细胞化学之间没有本质区别。一抗的特异性是可靠免疫细胞化学的最重要前提。酶免疫测定、免疫印迹、抗原斑点试验和组织检测是推荐的特异性分析方法。对最常用连接系统的研究表明，它们都适用于与单克隆抗体联合使用。这也适用于最重要的标记物。得出的结论是，基于单克隆抗体的免疫细胞化学是细胞生物学和病理学中最有价值的工具之一。
