Serdiuk O A, Tsytsikov E N, Vasilov R G
Biull Eksp Biol Med. 1989 Jun;107(6):722-4.
Enzyme immunoassay for determination of total immunoglobulin E in children and adolescents based on combination of two monoclonal antibodies with high affinity and specificity to different epitopes of IgE molecule has been developed. Monoclonal antibodies IgE/11-4 are used for sensitization of a solid phase (96-well plate), mcAB IgE/11-1 conjugated with horseradish peroxidase are used as a developing reagent. The following analytical characteristics of a test-system are established: range of assay--1-100 IU/ml sensitivity--1 IU/ml; time of analysis--1.5 hour, sample volume in duplicates--30 microliters. Immunoassay is simple, reliable and can be used in the field of pediatrics and allergology.
基于两种对IgE分子不同表位具有高亲和力和特异性的单克隆抗体组合，开发了用于测定儿童和青少年总免疫球蛋白E的酶免疫测定法。单克隆抗体IgE/11-4用于固相（96孔板）致敏，与辣根过氧化物酶偶联的单克隆抗体IgE/11-1用作显色试剂。确定了该检测系统的以下分析特性：测定范围——1-100 IU/ml，灵敏度——1 IU/ml；分析时间——1.5小时，重复检测的样本体积——30微升。该免疫测定法简单、可靠，可用于儿科和过敏学领域。
