STK31/TDRD8是一种生殖细胞特异性因子，对小鼠的繁殖并非必需。
STK31/TDRD8, a germ cell-specific factor, is dispensable for reproduction in mice.
作者信息
Zhou Jian, Leu N Adrian, Eckardt Sigrid, McLaughlin K John, Wang P Jeremy
机构信息
Department of Animal Biology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, Pennsylvania, United States of America.
Research Institute at Nationwide Children's Hospital, Columbus, Ohio, United States of America.
出版信息
PLoS One. 2014 Feb 19;9(2):e89471. doi: 10.1371/journal.pone.0089471. eCollection 2014.
Tudor domain containing (Tdrd) proteins that are expressed in germ cells are divided into two groups. One group, consisting of TDRD1, TDRKH, TDRD9 and TDRD12, function in piRNA biogenesis and retrotransposon silencing, while the other group including RNF17/TDRD4 and TDRD5-7 are required for spermiogenesis. These Tdrd proteins play distinct roles during male germ cell development. Here, we report the characterization of STK31/TDRD8 in mice. STK31 contains a tudor domain and a serine/threonine kinase domain. We find that STK31 is a cytoplasmic protein in germ cells. STK31 is expressed in embryonic gonocytes of both sexes and postnatal spermatocytes and round spermatids in males. Disruption of the tudor domain and kinase domain of STK31 respectively does not affect fertility in mice. Our data suggest that the function of STK31 may be redundant with other Tdrd proteins in germ cell development.
在生殖细胞中表达的含 Tudor 结构域（Tdrd）的蛋白质分为两组。一组由 TDRD1、TDRKH、TDRD9 和 TDRD12 组成，在 piRNA 生物合成和逆转座子沉默中发挥作用，而另一组包括 RNF17/TDRD4 和 TDRD5 - 7 是精子发生所必需的。这些 Tdrd 蛋白在雄性生殖细胞发育过程中发挥着不同的作用。在此，我们报道了小鼠中 STK31/TDRD8 的特征。STK31 包含一个 Tudor 结构域和一个丝氨酸/苏氨酸激酶结构域。我们发现 STK31 是生殖细胞中的一种细胞质蛋白。STK31 在两性的胚胎生殖母细胞以及雄性出生后的精母细胞和圆形精子细胞中表达。分别破坏 STK31 的 Tudor 结构域和激酶结构域并不影响小鼠的生育能力。我们的数据表明，STK31 的功能在生殖细胞发育中可能与其他 Tdrd 蛋白冗余。
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